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<ol>
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<table><tbody><tr><td>10% Tween-20</td><td>Bio-Rad Laboratories</td><td>1662404</td><td>NA</td></tr><tr><td>20x PBS, pH7.4</td><td>Growcells.com</td><td>MRGF-6396</td><td>2.74M NaCl, 53.7 mM KCl, 20.3 mM Na2HPO4, 29.4 mM KH2PO4&nbsp;</td></tr><tr><td>20x SSC</td><td>Quality Biological</td><td>351-003-131</td><td>3M NaCl, 0.3M Sodium Citrate</td></tr><tr><td>37 &deg;C incubator</td><td>Fisher Scientific (Isotemp)</td><td>15-015-2633</td><td>NA</td></tr><tr><td>37 &deg;C water bath</td><td>Fisher Scientific</td><td>FSGPD15D</td><td>NA</td></tr><tr><td>Aluminum foil</td><td>Reynolds Wrap/Fisher Brand</td><td>15-078-291</td><td>NA</td></tr><tr><td>Antibody: Mouse-anti-Abrupt</td><td>DSHB</td><td>Abrupt; RRID: AB_528344&nbsp;</td><td>Dilution: 1:20</td></tr><tr><td>Antibody: Chicken anti-GFP</td><td>Millipore Sigma</td><td>Catalog# 06-896; RRID: AB-619712</td><td>Dilution: 1:500</td></tr><tr><td>Antibody: Rabbit anti-GFP</td><td>Life Technologies</td><td>#11122; RRID: AB_221569</td><td>Dilution: 1:500</td></tr><tr><td>Antibody: Rabbit anti-Dll</td><td>Genscript/Desplan lab</td><td>-</td><td>Dilution: 1:200</td></tr><tr><td>Antibody: Rat anti-NCadherin</td><td>DSHB</td><td>DN-Ex #8, RRID: AB_528121</td><td>Dilution: 1:50</td></tr><tr><td>Antibody: Rabbit anti-SoxN</td><td>Genscript/Desplan lab</td><td>-</td><td>Dilution: 1:200</td></tr><tr><td>Antibody: Guinea Pig anti-Traffic jam</td><td>Gift from D. Godt</td><td>-</td><td>Dilution: 1:2,000</td></tr><tr><td>Antibody: Donkey anti-Rat 405</td><td>Jackson ImmunoResearch</td><td>Code # 712-475-150; RRID: AB_2340680</td><td>Dilution: 1:100</td></tr><tr><td>Antibody: Donkey anti-Chicken 488</td><td>Jackson ImmunoResearch</td><td>Code #703-545-155; RRID: AB_2340375</td><td>Dilution: 1:200</td></tr><tr><td>Antibody: Donkey anti-Mouse 488</td><td>Jackson ImmunoResearch</td><td>Code #715-545-151; RRID: AB_2341099</td><td>Dilution: 1:200</td></tr><tr><td>Antibody: Donkey anti-Rabbit 488</td><td>Jackson ImmunoResearch</td><td>Code #711-545-152; RRID: AB_2313584</td><td>Dilution: 1:200</td></tr><tr><td>Antibody: Donkey anti-Rabbit A555</td><td>Thermo Scientific</td><td>Catalog #A-31572; RRID: AB_162543</td><td>Dilution: 1:200</td></tr><tr><td>Antibody: Donkey anti-Rat Cy3</td><td>Jackson ImmunoResearch</td><td>Code #712-165-153; RRID: AB_2340667</td><td>Dilution: 1:200</td></tr><tr><td>Antibody: Donkey anti-Guinea Pig 647</td><td>Jackson ImmunoResearch</td><td>Code #706-605-148; RRID: AB_2340476</td><td>Dilution: 1:200</td></tr><tr><td>Antibody: Donkey anti-Rat 647</td><td>Jackson ImmunoResearch</td><td>Code #712-605-153; RRID: AB_2340694</td><td>Dilution: 1:200</td></tr><tr><td>Antifade mountant</td><td>Thermo Fisher Scientific</td><td>S36940 (SlowFade Gold Antifade Mountant)</td><td>Other slowfades will work as well, including ones with DAPI (S36936)</td></tr><tr><td>Confocal Microscope</td><td>Zeiss</td><td>LSM-880</td><td>NA</td></tr><tr><td>Cover slips (22 x 30)</td><td>VWR</td><td>16004-332</td><td>NA</td></tr><tr><td>Fly: D. melanogaster: ab-GFP-FLAG</td><td>BDSC</td><td>38626; RRID: BDSC_38626</td><td>NA</td></tr><tr><td>Fly: D. melanogaster: Canton S</td><td>BDSC</td><td>64349; RRID: BDSC_64349</td><td>NA</td></tr><tr><td>Fly: D. melanogaster: dpp-Gal4</td><td>BDSC</td><td>1553; RRID: BDSC_1553</td><td>NA</td></tr><tr><td>Fly: D. melanogaster: ham-GFP.FPTB</td><td>BDSC</td><td>83660; RRID: BDSC_83660</td><td>NA</td></tr><tr><td>Fly: D. melanogaster: yw; 10xUAS-myr-GFP;</td><td>BDSC</td><td>32198; RRID: BDSC_32198</td><td>NA</td></tr><tr><td>Fly: D. melanogaster: UAS-Stinger</td><td>BDSC</td><td>84277; RRID: BDSC_84277</td><td>NA</td></tr><tr><td>Fly: D. melanogaster:&nbsp; wit-KO-Gal4</td><td>BDSC</td><td>600136; RRID: BDSC_600136</td><td>NA</td></tr><tr><td>Forceps</td><td>Dumont</td><td>#5 or #55s</td><td>NA</td></tr><tr><td>Glass slides</td><td>VWR</td><td>16005-106</td><td>NA</td></tr><tr><td>Glycine</td><td>Millipore Sigma</td><td>G7126-10MG</td><td>NA</td></tr><tr><td>HCR Amplification buffer</td><td>Molecular Instruments</td><td>HCR Gold RNA-FISH Kit</td><td>NA</td></tr><tr><td>HCR-Gold Amplifiers</td><td>Molecular Instruments</td><td>HCR Gold RNA-FISH Kit</td><td>NA</td></tr><tr><td>HCR HiFi Probes</td><td>Molecular Instruments</td><td>HCR Gold RNA-FISH Kit</td><td>NA</td></tr><tr><td>HCR Probe Hybridization Buffer</td><td>Molecular Instruments</td><td>HCR Gold RNA-FISH Kit</td><td>Contains formamide</td></tr><tr><td>HCR Probe Wash Buffer</td><td>Molecular Instruments</td><td>HCR Gold RNA-FISH Kit</td><td>Contains formamide</td></tr><tr><td>Nail polish (clear)</td><td>Electron Microscopy Sciences</td><td>72180</td><td>NA</td></tr><tr><td>Nuclease-free water (not DEPC-treated)</td><td>Thermo Fisher Scientific</td><td>AM9937</td><td>NA</td></tr><tr><td>Nutating mixer</td><td>Corning/Fisher Scientific</td><td>10-320-100</td><td>NA</td></tr><tr><td>Paraformaldehyde, 16% (w/v)</td><td>Electron Microscopy Sciences</td><td>15710</td><td>Toxic</td></tr><tr><td>PCR Machine/Thermal Cycler</td><td>Bio-Rad</td><td>1851148 (C1000 Touch Thermal Cycler)</td><td>NA</td></tr><tr><td>PCR pull-apart tube strips with dome cap strips (0.2 mL)&nbsp;</td><td>USA Scientific</td><td>#1402-2400</td><td>NA</td></tr><tr><td>Petri dishes (150 x 15 mm; for making dissecting dishes)</td><td>Fisher Scientific</td><td>FB0875714</td><td>NA</td></tr><tr><td>RNase ZAP</td><td>ThermoFisher Scientific</td><td>AM9780</td><td>NA</td></tr><tr><td>Slide spacer stickers</td><td>SUNJin</td><td>IS016 (iSpacer stickers (0.2 mm deep))</td><td>NA</td></tr><tr><td>Sylgard elastomer kit (for making dissecting dishes)</td><td>Fisher Scientific</td><td>NC9285739</td><td>NA</td></tr><tr><td>Triton X-100</td><td>Merck Millipore Sigma</td><td>X100</td><td>NA</td></tr></tbody></table>

発達中の ショウジョウバエ の視葉におけるハイブリダイゼーション連鎖反応fishの

Hybridization Chain Reaction FISH in the Developing Drosophila Optic Lobe

発達中の ショウジョウバエ の視葉におけるハイブリダイゼーション連鎖反応FISHの

Understanding neural development requires precise characterization of when and where genes are expressed. Techniques such as in situ hybridization have ...

hybridization-chain-reaction-fish-developing-drosophila-optic

Research

JoVE Journal

Neuroscience

神経科学

全マウントを使用するその場で</em分子とOrganismal生物学をリンクする>ハイブリダイゼーション

の形態素解析ショウジョウバエ</em遺伝モザイクを使用>幼虫末梢感覚ニューロンの樹状突起と軸索

ここでは、ホールマウント蛍光を記述...

ホールマウントRNAを蛍光<em&gt;その場で</emの&gt;ハイブリダイゼーション<em&gt;ショウジョウバエ</em&gt;胚

ゼブラフィッシュは、小さな熱帯魚は、脊椎動物の発生と疾患の間に遺伝子機能を研究するための人気のあるモデルとなっている。標的遺伝子の時間的および空間的な表現は次式で求めることができる...

高解像度全マウント<em&gt;その場で</em遺伝子発現と機能を研究するためにゼブラフィッシュ胚内&gt;ハイブリダイゼーション

免疫染色は、組織内の特定の細胞タイプやタンパク質を可視化するための効果的な技術です。超音波処理を利用することにより、ここに記載されているプロトコルは、免疫染色の前に後期胚および幼虫からショウジョウバエ・メラノガスター組織を解剖する必要性を軽減する。私たちは、ホルムアルデヒド固定全マウント幼虫の免疫染色のための効率的な方法を提供します。...

後期胚および幼虫の超音波処理により促進免疫蛍光染色<em&gt;ショウジョウバエ</em&gt;組織<em&gt;その場で</em

我々は、色の沈殿物を対照することによって三つの異なるmRNAの分布パターンの同時および特定の検出を可能にする、完全なショウジョウバエの胚におけるマルチターゲット発色性ホールマウントin situハイブリダイゼーション （MC-WISH）の手順を説明します。...

マルチターゲット発色ホールマウント<em&gt;その場で</em&gt;ハイブリダイゼーションにおける遺伝子発現のドメインを比較します<em&gt;ショウジョウバエ</em&gt;胚

記載されている RNAその場で交配のプロトコルでは、全体のショウジョウバエの胚または切り裂かれたティッシュで RNA の検出をことができます。96 ウェル マイクロ...

ショウジョウバエの胚とパスチラミドシグナル信号増幅を利用した生体表面の高解像度蛍光In Situハイブリダイゼーション

この原稿は、 Xを生成するための詳細な方法を提示-染色体腕プローブおよび実行する蛍光その場で交配 (魚) の妹の状態を調べるに染色分け体結束の prometaphase...

Prometaphase の腕凝集度と中期の状態を監視する蛍光In Situハイブリダイゼーション (魚) を使用して私はショウジョウバエの卵母細胞

本稿では、ハイブリダイゼーション、連鎖反応、RNA、ホールマウント蛍光 in situ ハイブリダイゼーション(HCR、RNA、WM-FISH)を行い、蚊の触角と上顎触覚における化学感覚受容体遺伝子の空間的および細胞的分解能に関する洞察を明らかにするために必要な方法と試薬について説明します。...

ハイブリダイゼーション連鎖反応RNAホールマウント蛍光 蚊の嗅覚付属器における化学感覚遺伝子の in situ ハイブリダイゼーション

Expansion-Assisted Iterative Fluorescence In Situ Hybridization(EASI-FISH)は、拡大顕微鏡法と蛍光in...

Expansion-assisted iterative Fluorescence In Situ Hybridizationを用いたInsact Drosophila Brainにおける遺伝子発現のマルチプレックス検出

New York University.  このプロトコルは、ショウジョウバエのメラノガスター幼虫視葉におけるハイブリダイゼーション連鎖反応蛍光性交配(HCR-FISH)を行うために必要な手順を説明しています。プローブ設計、ハイブリダイゼーション、増幅、組織マウントの手順が詳細に記載され、発達中の視葉の可視化を容易にします。

発達中のショウジョウバエの視葉におけるハイブリダイゼーション連鎖反応FISHの

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発達中の ショウジョウバエ の視葉におけるハイブリダイゼーション連鎖反応FISHの

さらに動画を探す

発達中のショウジョウバエの視葉におけるハイブリダイゼーション連鎖反応FISHの