Name: ir spectrometer signal paths
Uploaded: 2024-12-05T18:32:08.000Z
Duration: 1 min 25 s
Channel: JoVE

분산형 적외선 분광계에서 열선에 의해 생성된 방사선은 미러에 의해 기준 셀과 샘플 셀을 통과하는 두 개의 평행한 동일 강도 빔으로 나뉩니다.
모노크로메이터는 빔을 천천히 회전하는 회절 격자 프리즘을 향해 교대로 통과하는 빔 초퍼로 구성되며, 이는 방사선의 주파수 또는 파장을 변화시킵니다.
열전대 감지기는 두 빔의 강도 비율을 감지합니다.
마지막으로 검출기의 신호가 증폭되고 레코더가 스펙트럼을 그립니다.
푸리에 변환 적외선 분광계에는 간섭계가 포함되며, 여기서 방사선은 샘플에 도달하기 전에 빔 스플리터, 고정 거울 및 이동 거울을 통과합니다.
빔 스플리터는 빔을 편향 및 편향되지 않은 방사선으로 분리하며, 이는 각각 고정 미러와 움직이는 미러에 떨어질 수 있습니다.
두 개의 빔은 스플리터에서 재결합하여 광범위한 파장 또는 주파수를 포함하는 간섭 패턴 또는 간섭도를 생성합니다.
검출기는 샘플에 의해 수정된 인터페로그램을 감지하고, 컴퓨터는 푸리에 변환 연산을 사용하여 개별 주파수를 추출합니다.

ir 분광기

IR Spectrometers

두 가지 주요 적외선(IR) 분광 광도계가 있습니다. 분산 IR 분광기와 푸리에 변환 적외선(FTIR) 분광기입니다. 분산 IR 분광기에서 열선에 의해 생성된 적외선 복사선 빔은 거울을 사용하여 두 개의 평행한 동일 강도 빔으로 나뉩니다. 한 빔은 표본을 통과하고 다른 빔은 참조 빔입니다. 그런 다음 빔은 모노크로메이터를 통과하여 복사선을 서로 다른 주파수의 연속 스펙트럼으로 분리합니다. 모노크로메이터는 회절 격자에 도달하기 전에 표본과 참조 빔을 번갈아 가며 빠르게 회전하는 빔 초퍼로 구성됩니다. 그런 다음 복사선은 두 빔의 강도 간의 비율을 측정하는 열전대 검출기를 통과합니다. 검출기의 신호가 증폭되고 결과 스펙트럼이 레코더를 사용하여 그려집니다. 분산 IR 분광기는 주파수 영역에서 스펙트럼을 기록합니다. 즉, 회절 격자의 회전에 따라 복사선의 주파수가 변함에 따라 스펙트럼이 캡처됩니다.

FTIR 분광기에서 소스에서 생성된 방사선은 빔 분할기를 통과합니다. 빔 분할기는 들어오는 방사선에 대해 45° 각도로 배치된 거울로, 빔을 굴절된 방사선과 굴절되지 않은 방사선으로 분리합니다. 이러한 방사선은 나중에 고정된 거울과 움직이는 거울에 떨어지도록 합니다. 두 거울의 움직임은 빔의 경로 길이를 변경하고 거울에서 반사된 방사선은 빔 분할기에서 결합된다는 점에 유의하는 것이 중요합니다. 따라서 생성된 결합 빔에는 방사선 경로 길이의 차이로 인해 생성 간섭 패턴과 파괴 간섭 패턴이 모두 포함됩니다.

<ul style="list-style-type: disc; margin-left: 35px;">
	<li>생성 간섭은 두 파동의 피크가 일치하여 결합된 파동의 진폭이 증가할 때 발생합니다.</li>
	<li>파괴 간섭은 한 파동의 피크가 다른 파동의 트로프와 일치하여 서로를 상쇄하고 진폭이 감소할 때 발생합니다.</li>
</ul>

간섭도라고 하는 결과 결합 빔은 검출기를 통과합니다. 검출기는 표본에 의해 수정된 간섭도를 검출하고, FT 프로세스는 간섭도에서 개별 흡수 주파수를 분리합니다.

FTIR 분광기는 더 빠른 작동과 더 높은 감도로 인해 분산 IR 분광기보다 선호됩니다.

IR 분광기

주요 적외선(IR) 분광광도계에는 분산형 IR 분광계와 푸리에 변환 적외선(FTIR) 분광계의 두 가지가 있습니다. 분산 IR에서 ...

Education

교육

JoVE Core

Analytical Chemistry

분석 화학

Unit 1

제1단원

Molecular Vibrational Spectroscopy

분자 진동 분광학

KEY TERMS AND CONCEPTS

주요 용어 및 개념

ir spectrometer signal paths

IR spectrometers use infrared light to compare a sample beam with a reference beam and record how each one behaves. The two main instruments are dispersive IR spectrometers and Fourier transform infrared, or FTIR, spectrometers.

In a dispersive IR spectrometer, a hot wire produces the infrared beam. Mirrors split it into two equal beams. One beam passes through the sample, and the other serves as the reference. The beams then move into the monochromator, which separates the radiation into a continuous range of frequencies.

The monochromator uses a rapidly rotating beam chopper and a diffraction grating. The chopper alternates the sample and reference beams before they reach the grating. After that, a thermocouple detector measures the ratio of the two beam intensities. The detector signal is amplified, and a recorder draws the spectrum. Dispersive IR spectrometers record data in the frequency domain, so the spectrum is captured as the grating rotates and the frequency changes.

FTIR spectrometers follow a different path. The source radiation passes through a beam splitter, which is a mirror placed at a 45° angle to the incoming beam. The splitter divides the beam into deflected and undeflected parts. These parts fall on fixed and moving mirrors, and the changing mirror motion changes the path length of the beams. When the beams return to the beam splitter, they combine and produce both constructive and destructive interference.

Constructive interference happens when wave peaks line up. Destructive interference happens when a peak meets a trough and the waves partly cancel. The combined beam is called an interferogram. It then passes through the detector, which measures the interferogram after it has been modified by the sample. The FT process separates the individual absorption frequencies from that pattern. FTIR spectrometers are often preferred because they work faster and are more sensitive than dispersive IR spectrometers.

IR Spectrometer Signal Paths

infrared (ir) spectroscopy: overview

IR Spectra and Functional Group ID

Infrared (IR) spectroscopy helps identify functional groups in molecules by measuring how they absorb infrared light. It is based on the idea that electromagnetic radiation can move atoms or molecules from a lower energy state to a higher one when the radiation matches the energy gap between those states. In IR spectroscopy, that absorption happens as molecular vibrations change.

ir spectroscopy: molecular vibration overview

ir-spectroscopy-molecular-vibration-overview

IR Spectroscopy: Vibration Types and Bands

IR spectroscopy explains how covalently bonded molecules absorb infrared radiation by moving to higher vibrational levels. The key motions are stretching and bending vibrations. These motions help describe how a bond responds when it absorbs IR energy. Stretching vibrations happen along the bond line and change the distance between two bonded atoms. They can be symmetric, where two bonds lengthen and shorten together, or asymmetric, where one bond moves one way while another moves the opposite...

ir spectroscopy: hooke's law approximation of molecular vibration

ir-spectroscopy-hooke-s-law-approximation-of-molecular-vibration

Molecular Bond Vibrations in IR Spectroscopy

Molecular bond vibrations can be modeled with IR spectroscopy using Hooke’s law. A covalently bonded heteronuclear diatomic molecule is treated like two masses connected by a spring. The atoms act like the masses, and the bond acts like the spring. Hooke’s law links the vibrational frequency to two main factors. The frequency increases with the force constant, K, which describes bond stiffness. It decreases with the reduced mass, μ, which combines the masses of both atoms into one value for...

ir spectrometers

IR spectrometers use infrared light to compare a sample beam with a reference beam and record how each one behaves. The two main instruments are dispersive IR spectrometers and Fourier transform infrared, or FTIR, spectrometers. In a dispersive IR spectrometer, a hot wire produces the infrared beam. Mirrors split it into two equal beams. One beam passes through the sample, and the other serves as the reference. The beams then move into the monochromator, which separates the radiation into a...

ir spectrum

IR Spectrum Peak Regions and Patterns

Infrared (IR) spectroscopy shows how a molecule responds when IR radiation passes through it. The bonds in the molecule stretch or bend as they absorb that energy. This creates an absorption spectrum, which plots percent transmittance against wavenumber. Transmittance is the ratio of radiant power that passes through a sample to the radiant power from the source. When transmittance is multiplied by 100, it becomes percent transmittance, or %T. %T ranges from 100% for no absorption to 0% for...

ir absorption frequency: hybridization

C-H Stretching in Alkane, Alkene, and Alkyne IR

Hydrocarbons such as alkanes, alkenes, and alkynes show characteristic C–H stretching bands in infrared, or IR, spectra. These bands help identify how the carbon atoms are hybridized. The bond type affects the stretching frequency that appears in the spectrum. The difference comes from s character, which is the amount of s-orbital contribution in a hybrid orbital. Among sp, sp2, and sp3 hybridized orbitals, sp has the most s character at 50%. Because of that, the electrons are held closer to...

ir absorption frequency: delocalization

Conjugation Shifts IR Bond Stretching

Conjugation shifts IR bond stretching because electron delocalization changes bond strength. Electron delocalization means electrons spread across more than one atom instead of staying in one bond or one atom. It is common in conjugated systems, where alternating single and double bonds let pi-electrons move through the molecule. This electron movement helps stabilize the molecule. It also lowers the double bond character of the bonds in the conjugated network. When a bond has less double bond...

ir frequency region: x&ndash;h stretching

IR Peaks for C-H, O-H, and N-H Bonds

IR spectroscopy shows the stretching bands of C-H, O-H, and N-H bonds in the 2700-4000 cm-1 range. These X-H bonds are useful for identifying common functional groups in a spectrum. Their peaks appear in different parts of this region and often have different shapes and strengths. C-H stretching gives sharp bands in the 2850-3000 cm-1 range. O-H stretching appears in the 3650-3200 cm-1 range, and N-H stretching appears in the 3500-3100 cm-1 range. N-H stretching usually shows one or two sharp...

ir frequency region: alkyne and nitrile stretching

ir-frequency-region-alkyne-and-nitrile-stretching

Triple-Bond Signals in IR Spectra

Alkyne and nitrile groups both contain triple bonds, and they show clear stretching bands in the diagnostic IR region. These absorptions usually appear around 2100 to 2300 cm −1 . This makes them useful markers when reading an IR spectrum. Triple bonds absorb at a higher stretching frequency than double bonds and single bonds. The C≡C bond stretches at a higher frequency than a C=C or C–C bond. The C≡N bond also stretches at a higher absorption than C=N and C–N bonds. This higher stretching...

ir frequency region: alkene and carbonyl stretching

ir-frequency-region-alkene-and-carbonyl-stretching

IR Signals for C=C and C=O Bonds

IR spectroscopy helps identify alkene double bonds and carbonyl groups by their stretching signals. These bonds absorb in a diagnostic region of the IR spectrum. Alkenes also show vinylic C–H stretching and C–H out-of-plane bending bands. Those bands can help determine substitution patterns on the double bond. The exact stretching frequency can change with bond environment. Resonance, inductive effects, ring strain, dipole moment, and hydrogen bonding can all shift the absorption. That is why...

ir frequency region: fingerprint region

IR Spectra: Diagnostic vs Fingerprint Region

IR spectra are divided into a diagnostic region and a fingerprint region. The diagnostic region lies above 1500 cm −1 and appears on the left side of the spectrum. It shows key bond stretches that help identify functional groups. In this region, single-bond vibrations from N-H, C-H, and O-H stretches appear at higher wavenumbers. The C≡C and C≡N stretches occur between 2100 and 2300 cm −1. The C=O, C=N, and C=C stretches appear between 1600 and 1850 cm −1. The fingerprint region lies below...

ir spectrum peak intensity: amount of ir-active bonds

ir-spectrum-peak-intensity-amount-of-ir-active-bonds

IR Peak Intensity and Bond Dipole Change

IR peak intensity depends on how much a bond’s dipole moment changes during vibration. When infrared light passes through a molecule, absorption happens only when a stretching or bending motion causes a substantial change in that bond dipole moment. That is what makes a molecule infrared active. Vibrational transitions usually fall in the infrared region from 4000 to 400 cm-1. Molecular bonds can stretch and bend in different ways, so an IR spectrum shows peaks with different intensities. The...

ir spectrum peak intensity: dipole moment

IR Absorption and Bond Dipole Changes

IR absorption depends on whether a bond has a changing dipole moment. A dipole moment comes from partial charge on each atom and the distance between them. It helps determine how strongly a bond absorbs infrared light and how intense the peak looks. A bond in an electric field can be stretched or compressed depending on the field direction. Infrared radiation has an electric field that flips direction very quickly. So polar bonds are pulled back and forth as the field reverses. If this motion...

ir spectrum peak broadening: hydrogen bonding

ir-spectrum-peak-broadening-hydrogen-bonding

Hydrogen Bonding Effects in IR O-H Peaks

Hydrogen bonding changes the O-H stretching peak in IR spectroscopy. The bond’s vibrational frequency depends on bond strength. Stronger bonds vibrate at higher frequencies, while weaker bonds vibrate at lower frequencies. In very dilute alcohol or phenol samples, or in the gas phase, the strong O-H bond gives a sharp peak at 3600-3650 cm−1. When intermolecular hydrogen bonding or intramolecular hydrogen bonding weakens the O-H bond, the stretch shifts to about 3300-3400 cm−1. This range of...

ir spectrum peak splitting: symmetric vs asymmetric vibrations

ir-spectrum-peak-splitting-symmetric-vs-asymmetric-vibrations

IR Peak Splitting in Amines and Nitro Groups

IR peak splitting can reveal how identical bonds in a polyatomic group are moving. These bonds may stretch in phase, which is symmetric stretching, or out of phase, which is asymmetric stretching. The two motions often create different IR peak shapes and positions. As a general rule, asymmetric stretching appears at a higher frequency than symmetric stretching. This pattern is useful for spotting functional groups in an IR spectrum. It also helps explain why some groups show one peak while...

applications of ir spectroscopy: overview

IR Spectroscopy Uses in Science and Forensics

IR spectroscopy is a useful tool for finding out what materials are made of. It is non-destructive, so the sample can often be kept intact. It gives chemical information about functional groups and the bonds between atoms in a molecule. This makes IR spectroscopy helpful in organic synthesis, pharmaceutical research, and materials science. Scientists use it to identify and characterize organic and inorganic compounds. It also helps with structural elucidation, which means working out a...

attenuated total reflectance (atr) infrared spectroscopy: overview

attenuated-total-reflectance-atr-infrared-spectroscopy-overview

ATR Infrared Spectroscopy for Material ID

ATR infrared spectroscopy is a method for identifying the composition of materials. It is used in chemistry, materials science, forensic science, and other areas where sample characterization is needed. The technique is useful because it usually needs little to no sample preparation and can be applied to many kinds of samples. The process starts when infrared, or IR, radiation is directed onto a diamond or germanium crystal. Because this crystal has a high refractive index, the IR beam...

raman spectroscopy: overview

Raman Spectra and Molecular Fingerprints

Raman spectroscopy uses laser light to study how molecules scatter light and reveal their vibrational energy levels. A monochromatic beam, or single-color light, hits the sample first. Most of the scattered light keeps the same frequency as the incoming light. This is called Rayleigh scattering. A small part of the light shifts in frequency because the photons exchange energy with molecular vibrations. That shifted light is Raman scattering. To collect a Raman spectrum, the sample is...

raman spectroscopy instrumentation: overview

raman-spectroscopy-instrumentation-overview

Raman Spectroscopy Setup and Detectors

Raman spectroscopy uses a laser, a sample holder, a wavelength selector, and a detector to measure scattered light from a sample. The laser is monochromatic, meaning it has one main wavelength. It usually uses visible or near-infrared light to create a tightly focused beam. That beam hits the sample and some of the light scatters. Liquid and gas samples are often placed in ordinary glass capillaries. Solid samples can be tested as powders packed into capillaries or as potassium bromide pellets.

key terms and concepts

Compare dispersive IR and FTIR spectrometers, from beam splitting and monochromators to interferograms, detectors, and why FTIR is faster and more sensitive.

IR Spectrometer Signal Paths | JoVE K12

analytical chemistry

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IR Spectrometer Signal Paths

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