Name: chromatid pulling in cell division
Uploaded: 2023-04-30T18:28:50.000Z
Duration: 2 min 17 s
Channel: JoVE

K12 Standards

Pennsylvania State Academic Standards

9-12

Life Science

Inheritance and Variation of Traits

3.1.9-12.D

Separation of Sister Chromatids

중기가 끝나면 이중 배향 염색체가 중기 플레이트에 정렬됩니다. 이 단계에서 코헤신 단백질 고리 복합체는 중심체 영역에서 자매 염색체를 함께 유지하고 분리되는 것을 방지합니다.
중기에서 아나페이즈로의 진행은 사이클린-Cdk 유도 인산화에 의해 촉발됩니다 – 사이클로솜 또는 APC/C라고도 하는 아나페이즈 촉진 복합체인 유비퀴틴 리가아제 효소(cyclin-Cdk 유도 인산화)는 단백질인 Cdc20에 결합하여 활성 복합체를 형성합니다.
활성 APC/C 복합체는 세파라아제(separase)라고 하는 프로테아제 효소에 결합된 세큐린(securin)이라는 억제 단백질을 인식합니다. 이 인식 이전에, securin는 separase의 활동을 금합니다.
활성 APC/C 복합체는 유비퀴틴(ubiquitin) 단백질과 함께 세큐린(securin)을 태그하여 프로테아솜 분해를 표적으로 합니다. securin의 파괴는 separase를 방출합니다.
cyclin-Cdk 복합체는 또한 억제성 인산화를 통해 결합되지 않은 분리효소 활성을 부정적으로 조절합니다.
활성 APC/C 복합체는 cyclins의 유비퀴틴화를 유발하여 프로테아좀 분해를 표적으로 합니다. cyclin의 파괴는 cyclin 의존하는 kinases 또는 Cdks의 효소 활동을 제거합니다.
Cdks의 불활성화는 인산가수분해효소가 separase를 dephosphorylate하도록 하고 separase의 Cdk 매개 억제성 재인산화를 방지합니다. 탈인산화는 분리효소가 자매 염색분체를 함께 유지하는 코헤신 고리 복합체를 절단할 수 있도록 합니다.
응집체 분열은 자매-염색체 응집력의 절대 상실을 초래하여 중기에서 아나페이즈로의 전환을 표시합니다. 응집력의 상실은 자매 염색체가 분리되어 방추체의 반대쪽 극으로 이동할 수 있도록 합니다.

<ol>
	<li>클레어 아쿠아비바, 조나단 파인스. 20016. "아나페이즈 촉진 복합체/사이클로솜: APC/C". 세포 과학 저널. 119: 2401-2404. [<a href="https://jcs.biologists.org/content/119/12/2401#sec-2">출처</a>]</li>
	<li>프랑크 울만(Frank Uhlmann), 프리드리히 로츠페이히(Friedrich Lottspeich), 킴 나스미스(Kim Nasmyth). 1999. "아나페이즈 발병 시 자매-염색체 분리는 코헤신 소단위 scc1의 절단에 의해 촉진됩니다." Nature 400 (6739). 네이처 퍼블리싱 그룹: 37–42. [<a href="https://mcb.berkeley.edu/courses/mcb230/WEB/PAPERS/SCC1.pdf">출처</a>]</li>
	<li>Farr, Katie A. 및 Orna Cohen-Fix. "중기에서 아나페이즈로의 전환: 생산적 파괴의 사례." 유럽 생화학 저널 263, no. 1 (1999): 14-19.</li>
	<li>Fang, Guowei, Hongtao Yu 및 W. Kirschner. "anaphase-promoting complex에 의한 유사 분열 전이 제어." 런던 왕립 학회의 철학적 거래. 시리즈 B : 생물 과학 354, no. 1389 (1999) : 1583-1590.</li>
</ol>

자매 염색분체의 분리

전기에서 중기로의 전환에서는 염색체 팔을 따라 응집력이 감소하여 자매 염색체가 분리됩니다. 그러나 잔류 코헤신 연결은 중기에서 후기로 전환될 때까지 자매 염색분체를 함께 유지하기 위해 남아 있습니다. 잔여 연결은 자매 염색체의 조기 분리를 방지하여 딸 세포 내의 이수성 위험을 차단합니다.

후기 단계가 시작되면 단백질 분해 효소인 분리효소가 활성화됩니다. 활성화된 분리효소는 잔류 코헤신 고리의 Scc1 하위 단위를 절단하여 응집력을 완전히 상실합니다. 자매염색분체는 이를 함께 묶어줄 힘이 없으면 분리됩니다. 응집력이 부족하면 미세소관을 따라 긴장되는 극방향 힘이 분리된 염색분체를 방추극 쪽으로 끌어당길 수 있습니다.

초기 유사분열에서 방추 조립 체크포인트(SAC)는 후기 촉진 복합체 또는 사이클로솜(APC/C)이 후기에 분해가 필요한 세쿠린 및 M상 사이클린과 같은 단백질을 유비퀴틴화하는 것을 금지합니다. 각 염색체가 유사분열 방추에 올바르게 정렬된 경우에만 SAC가 비활성화되어 APC/C 조절 하위 단위인 CDC20 및 CDH1의 인산화 및 활성화가 허용됩니다.

CDC20은 Cdk1/cyclin B에 의해 인산화되어 활성 APC/C-CDC20을 형성합니다. 활성 APC/C-CDC20은 securin, cyclin A 및 cyclin B의 분해를 촉매하여 후기 전이를 촉진합니다. Cdk 활성은 cyclin에 의존하기 때문에 cyclin B 분해로 인해 Cdk1 활성이 손실됩니다. Cdk1/cyclin B 복합체의 손실은 APC/C-CDC20을 비활성화하지만 중기에서 후기로의 전환 완료를 알리는 또 다른 조절 하위 단위인 APC/C-CDH1을 활성화합니다. 활성 APC/C-CDH1은 유사분열 사이클린의 조기 축적을 방지하여 유사분열 종료를 촉진하고 후속 G1 단계를 안정화합니다.

APC/C-CDC20 및 APC/C-CDH1 활동의 전환은 두 가지 중요한 결과를 가져옵니다. 첫째, 이러한 조절 하위 단위는 중복되지만 뚜렷한 기질 특이성을 유발하여 질서 있는 세포 주기 전환을 촉진합니다. 둘째, CDC20과 CDH1은 서로 다른 메커니즘을 통해 규제됩니다. APC/C-CDC20이 활성화되면 CDH1은 cyclin B/Cdk1에 의해 억제성 인산화를 거쳐 APC/C에 결합하는 것을 방지합니다. 대조적으로, CDC20 활성은 SAC에 의해 활성화되는 다중단백질(BUBR1, BUB3, CDC20 및 MAD2)인 유사분열 체크포인트 복합체에 의해 억제됩니다.

전단계(prophase)에서 중기(metaphase)로의 전환에서, 염색체팔을 따라 응집력이 감소하여 자매(sister)가 해결됩니다.

Education

교육

JoVE Core

Cell Biology

세포생물학

Unit 1

제1단원

Cell Division

세포 분열

KEY TERMS AND CONCEPTS

주요 용어 및 개념

chromatid pulling in cell division

Separation of sister chromatids is a key step in cell division. Sister chromatids are two identical copies of a chromosome that stay attached until the cell is ready to split them apart. Their separation helps each new cell receive the correct genetic material.

During this stage, the sister chromatids are pulled to opposite sides of the cell. This movement happens as the cell prepares to divide into two daughter cells. Once the chromatids separate, each side of the cell has a complete set of chromosomes.

This process is essential for accurate chromosome distribution. If sister chromatids do not separate properly, the new cells may not get the right genetic information. Understanding this step helps explain how cells copy and pass on DNA during division.

Chromatid Pulling in Cell Division

mitosis and cytokinesis

Stages of Cell Division in Eukaryotes

Cell division in eukaryotes follows a tightly regulated cycle that includes cell growth, DNA replication, chromosome duplication, chromosome distribution to daughter cells, and cell division. Regulatory systems and extracellular signals help control when cells divide. In typical human cells, the cycle takes about 24 hours. The cycle has two major stages. DNA is copied during the S phase of interphase. The mitotic, or M, phase then separates the duplicated chromosomes into two new nuclei and...

chromosome duplication

Chromosome Copying in Cell Division

Chromosome copying is an important step in cell division. Before a cell divides, it must make an exact copy of its chromosomes so each new cell can receive the same genetic information. A chromosome is a DNA-containing structure that carries genes. During duplication, the chromosome is copied into two identical sister chromatids. These copies stay attached until the cell is ready to separate them. This process helps ensure that daughter cells inherit a complete set of chromosomes. Accurate...

cohesins

Cohesins in Sister Chromatid Separation

Cohesins are protein complexes that help hold sister chromatids together after DNA is copied. They play a key role in cell division by keeping the two copies of a chromosome connected until the right time. These proteins act like a link between the sister chromatids. When the cell is ready to separate them, cohesins must be removed or released so each new cell can get one complete set of chromosomes. Cohesins are an important part of chromosome behavior during mitosis and meiosis. Their...

condensins

Condensin Proteins in Chromosome Packing

Condensin proteins help pack DNA into condensed chromosomes during cell division. They are part of the larger set of proteins that organize genetic material so it can be separated correctly when a cell divides. These proteins act by shaping and tightening chromosome structure. This process is important because DNA must be compacted before it can move through the stages of division. Condensins are therefore closely linked to chromosome condensation, the step that makes chromosomes more compact...

the mitotic spindle

How the Mitotic Spindle Guides Cell Division

The mitotic spindle guides chromosome movement during cell division. It is a structure made of microtubules, which are thin protein fibers in the cell. These fibers organize and help separate the genetic material before the cell splits. The spindle forms as the cell prepares to divide. Its fibers attach to chromosomes and line them up so they can be moved apart in an orderly way. This careful organization helps each new cell receive the correct set of chromosomes. The mitotic spindle is a key...

centrosome duplication

Centrosome Duplication in Cell Division

Centrosome duplication is an important step in cell division. The centrosome helps organize the cell’s internal structure, and it must be copied before a cell divides.

spindle assembly

Spindle Formation in Cell Division

Spindle formation in cell division is the process that builds the microtubule-based spindle needed to separate chromosomes. Microtubules are long protein fibers that help move and organize cell parts during division. During this process, microtubules grow and are arranged into a spindle structure around the chromosomes. The spindle forms in a way that supports chromosome movement to opposite sides of the cell. This step is essential for accurate cell division. Spindle formation depends on the...

attachment of sister chromatids

Sister Chromatid Attachment in Mitosis

Sister chromatid attachment in mitosis helps align duplicated chromosomes before they separate. Sister chromatids are the matching copies made after DNA replication. They stay connected until the cell is ready to divide. The attachment step is closely tied to the metaphase plate, the middle of the cell where chromosomes line up. This alignment helps each new cell receive one copy of every chromosome. Proper attachment is an important part of accurate cell division.

forces acting on chromosomes

Chromosome Movement During Cell Division

Chromosome movement during cell division depends on forces that act on the chromosomes. These forces help move the chromosomes into the right position as the cell divides. The transcript focuses on how these forces affect chromosome behavior during division. It highlights the role of the mitotic spindle, which is the structure that helps organize and move chromosomes. The spindle uses force to guide chromosome movement at the right time. Understanding these forces helps explain how...

separation of sister chromatids

Separation of sister chromatids is a key step in cell division. Sister chromatids are two identical copies of a chromosome that stay attached until the cell is ready to split them apart. Their separation helps each new cell receive the correct genetic material. During this stage, the sister chromatids are pulled to opposite sides of the cell. This movement happens as the cell prepares to divide into two daughter cells. Once the chromatids separate, each side of the cell has a complete set of...

the spindle assembly checkpoint

How Cells Verify Chromosome Spindle Attachment

The spindle assembly checkpoint helps cells verify that chromosomes are attached to the spindle before division continues. It acts as a safety step during cell division and helps prevent errors in chromosome separation. This checkpoint is controlled by kinetochores, which are protein structures on chromosomes that connect to spindle fibers. If a chromosome is not attached correctly, the checkpoint can delay progression until the problem is fixed. Once the attachment is correct, the cell can...

anaphase a and b

Chromosome and Spindle Movement in Anaphase

Anaphase is the stage of cell division when chromosomes move apart. In this phase, sister chromatids separate and travel toward opposite poles of the cell. Chromosome movement in anaphase can happen in two ways. In anaphase A, chromosomes move closer to the poles as the microtubules attached to them shorten. In anaphase B, the spindle poles themselves move farther apart and help stretch the cell. These two movements work together to ensure that the separated chromosomes are pulled to opposite...

anaphase promoting complex

Cell Cycle Control by APC

The anaphase-promoting complex, or APC, helps control cell cycle progression. It acts during cell division to help move the process from one stage to the next. This control is important for proper timing in the cell cycle. APC is a protein complex that works as a regulator. It helps trigger key steps that allow division to continue in an ordered way. By guiding this transition, APC supports normal cell cycle control.

the contractile ring

Cell Division and the Contractile Ring

The contractile ring helps cells divide at the end of cell division. It forms at the cell's center and tightens to split one cell into two. This process is part of cytokinesis, the stage when the cytoplasm divides. The ring is made of actin and myosin, two proteins that can work together to create force. As the ring contracts, it draws the cell membrane inward. This pinch helps separate the two daughter cells. Understanding the contractile ring is important for studying how cells complete...

determining the plane of cell division

Spindle Position and Cell Division Plane

The plane of cell division is set by the position of the spindle during mitosis. Cells use this internal structure to help decide where the cell will split. The result affects how the two daughter cells are arranged after division. This process is important in many developing tissues. It helps explain how cells divide in a controlled direction. A clear plane of division supports proper cell organization as new cells form. The transcript focuses on how the spindle is used to determine that...

the phragmoplast

Phragmoplast in Plant Cell Division

The phragmoplast is a plant cell structure that forms during cell division. It helps guide the final stage of division, when the cell begins to split into two daughter cells. In plant cells, the phragmoplast appears between the two new nuclei. It is part of the process that leads to the formation of a new cell wall. This wall separates the daughter cells after division is complete. The phragmoplast plays an important role in plant cytokinesis, which is the physical division of the cytoplasm.

distribution of cytoplasmic content

Cytoplasmic Content During Cell Division

Cytoplasmic content is distributed as a cell divides. This process helps each new cell receive the materials it needs to function. The cytoplasm includes the fluid and structures inside the cell membrane, but outside the nucleus. During cell division, this content is separated between the two daughter cells. The division must be organized so both cells can continue normal activity after the split. The video focuses on how this distribution occurs as part of cell division. It highlights the...

key terms and concepts

Learn how sister chromatids separate during cell division and move to opposite sides of the cell so daughter cells receive the correct genetic material.

Chromatid Pulling in Cell Division | JoVE K12

cell biology

JoVE Core Cell Biology illustrates complex cellular processes in easy-to-understand yet comprehensive animated video lessons. This collection focuses on the essential components and functions of cells, including organelles and membranes, cellular transport, and cell energetics. It is complemented by scientist-in-action videos that demonstrate the application of cell biology in real-world research experiments.

Louisiana Student Standards for Science

High School

Life Sciences

from-molecules-to-organisms-structures-and-processes

From Molecules To Organisms: Structures And Processes

HS-LS1-4

Tennessee Academic Standards

Biology

From Molecules to Organisms: Structures and Processes

BIO1.LS1.6

Minnesota Academic Standards in Science

3.1.1 9L.3.1.1.2

new-jersey-student-learning-standards-for-science

New Jersey Student Learning Standards for Science

Arizona Science Standards

HS+B.L1

HS+B.L1U1.9

Alabama Course of Study Science

9-12.B.1.3

Florida Department of Education

SC.912.L.16

SC.912.L.16.14

south-carolina-college-and-career-ready-science-standards

South Carolina College and Career Ready Science Standards

B-LS1-4.

What is the Cell Cycle?

The cell cycle refers to the sequence of events occurring throughout a typical cell&#8217;s life. In eukaryotic cells, the somatic cell cycle has two ...

Interphase

The cell cycle occurs over approximately 24 hours (in a typical human cell) and in two distinct stages: interphase, which includes three phases of the ...

The Cell Cycle Control System

Positive Regulator Molecules

Inhibition of CDK Activity

Mitosis and Cytokinesis

In eukaryotes, the cell division cycle is divided into distinct, coordinated cellular processes that include cell growth, DNA replication/chromosome ...

Chromosome Duplication

Cohesins

Condensins

The Mitotic Spindle

Centrosome Duplication

Spindle Assembly

Attachment of Sister Chromatids

Forces Acting on Chromosomes

The Spindle Assembly Checkpoint

Anaphase A and B

Anaphase Promoting Complex

The Contractile Ring

Determining the Plane of Cell Division

The Phragmoplast

Distribution of Cytoplasmic Content

An Introduction to Cell Division

Cell division is the process by which a parent cell divides and gives rise to two or more daughter cells. It is a means of reproduction for single-cell ...

Cell Cycle Analysis

Cell cycle refers to the set of events through which a cell grows, replicates its genome, and ultimately divides into two daughter cells through the ...

Live Cell Imaging of Mitosis

Mitosis is a form of cell division in which a cell&#8217;s genetic material is divided equally between two daughter cells. Mitosis can be broken down into ...

Fertilization

During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ...

Cleavage and Blastulation

After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage ...

Gastrulation

Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of ...

Neurulation

Neurulation is the embryological process which forms the precursors of the central nervous system and occurs after gastrulation has established the three ...

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms ...

an-introduction-to-developmental-genetics

An Introduction to Developmental Genetics

Development is the complex process through which a single-celled embryo transforms into a multicellular organism. Developmental processes are guided by ...

An Introduction to Organogenesis

Organogenesis is the process by which organs arise from one of three germ layers during the later stages of embryonic development. Researchers studying ...

Fate Mapping

Fate mapping is a technique used to understand how embryonic cells divide, differentiate, and migrate during development. In classic fate mapping ...

Students who demonstrate understanding can use a model to illustrate the role of cellular division (mitosis) and differentiation in producing and maintaining complex organisms.  Assessment Boundary: Assessment does not include specific gene control mechanisms or rote memorization of the steps of mitosis.

DNA Packaging

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The ...

Organization of Genes

Structure of a Gene

A gene is the fundamental unit of heredity. Every individual has two copies of each gene, one inherited from each parent. Although most people contain the ...

Chromosome Structure

A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA ...

Chromosome Replication

Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone ...

The Nucleosome

DNA in a human cell is almost 2m long and it is packed inside a tiny nucleus that is only a few microns in diameter. The level of compaction of DNA inside ...

The Nucleosome Core Particle

Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of ...

Nucleosome Remodeling

Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA ...

Chromatin Packaging

Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 ...

Karyotyping

Proteins: From Genes to Degradation

The DNA Helix

Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA ...

The Central Dogma

The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between ...

Mutations

Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations ...

genome-size-and-the-evolution-of-new-genes

Genome Size and the Evolution of New Genes

Gene Families

Students who demonstrate understanding can ask questions to clarify relationships about the role of DNA and chromosomes in coding the instructions for characteristic traits passed from parents to offspring. Assessment Boundary: Assessment does not include the phases of meiosis or the biochemical mechanism of specific steps in the process.

3.1.9-12.P

Genomic DNA in Prokaryotes

The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the ...

Binary Fission

Fission is the division of a single entity into two or more parts, which regenerate into separate entities that resemble the original. Organisms in the ...

Genomic DNA in Eukaryotes

Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the ...

What is Meiosis?

Meiosis is the process by which diploid cells divide to produce haploid daughter cells. In humans, each diploid cell contains 46 chromosomes, half from ...

Meiosis I

Meiosis is a carefully orchestrated set of cell divisions, the goal of which&#x2014;in humans&#x2014;is to produce haploid sperm or eggs, each containing ...

Meiosis II

Meiosis II is the second and final stage of meiosis. It relies on the haploid cells produced during meiosis I, each of which contain only 23 ...

Crossing Over

Unlike mitosis, meiosis aims for genetic diversity in its creation of haploid gametes. Dividing germ cells first begin this process in prophase I, where ...

Nondisjunction

During meiosis, chromosomes occasionally separate improperly. This occurs due to failure of homologous chromosome separation during meiosis I or failed ...

Students who demonstrate understanding can make and defend a claim based on evidence that inheritable genetic variations may result from (1) new genetic combinations through meiosis, (2) viable errors occurring during replication, and/or (3) mutations caused by environmental factors. Assessment Boundary: Assessment does not include the phases of meiosis or the biochemical mechanism of specific steps in the process.

3.1.9-12.Q

Chromatid Pulling in Cell Division

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