의 발달 프로세스 추적 및 정량화<em> C. 엘레</em> 오픈 소스 도구를 사용하여
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Summary
Abstract
Introduction
Protocol
Representative Results
Discussion
Disclosures
The authors have nothing to disclose.
Acknowledgements
Materials
| Stereo microscope | Motic/VWR | OT4005S | Stereo microscope for dissection and mounting |
| Polybead Polystyrene Microspheres, | Polysciences | 18329 | Embryo mounting |
| 20 µm | |||
| Polybead Polystyrene Microspheres, | Polysciences | 876 | Adult animal mounting |
| 0.1 µm | |||
| Microscope slides | VWR | 631-0902 | Adult animal mounting |
| Cover glass 18×18 mm | VWR | 631-1331 | Embryo/adult mounting |
| Cover glass 24×60 mm | VWR | 631-1339 | Embryo mounting |
| Scalpel | VWR | 233-5455 | Embryo dissection |
| Silicone tubing | VWR | 228-1501 | Tubing for mouth pipette |
| 30 mm PTFE membrane filter | NeoLab | Jul-01 | Filter for mouth pipette |
| Capillary tubes | VWR | 621-0003 | Pipette tip for mouth pipette |
| Vaseline | Roth | E746.1 | Embryo/adult mounting |
| Agar | Roth | 5210.5 | Adult animal mounting |
| Potassium-di-hydrogenphosphate | Roth | P018.2 | M9 buffer |
| Di-sodium- hydrogenphosphate | Roth | P030.2 | M9 buffer |
| Sodium chloride | Roth | 3957.1 | M9 buffer |
| VisiScope Spinning Disc Confocal System | Visitron Systems | n/a | Confocal microscopy |
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Cite This Article
Dutta, P., Lehmann, C., Odedra, D., Singh, D., Pohl, C. Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools. J. Vis. Exp. (106), e53469, doi:10.3791/53469 (2015).