Erratum: Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

요약

An erratum was issued for Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development.

The human RCTEC/RCTE cell stocks used here and in Caralt et al., 2015, Uzarski et al., 2015 were originally provided by Dr. Loghman-Adham (then at St. Louis University) to co-author Dr. Wandinger-Ness (University of New Mexico) through an MTA in 2001. The SV40 immortalized human RCTEC/RCTE cells were characterized as being of distal tubule cell line as detailed in Loghman-Adham et al., 2003. On the basis of recent short tandem repeat (STR) DNA sequencing (Performed by IDEXX BioResearch) of the earliest passages of the RCTEC/RCTE cell stocks it became evident the cells were of mixed lineage. Further analyses of PCR products using QIAxcel capillary electrophoresis demonstrated the presence of a canine product. The product was sequenced and established to be of canine origin. A set of canine specific STR markers were compared to the sample and showed that the sample had a genetic profile with 92% identity to the MDCK cell line.  Later cell stocks that were used in the present publication were STR profiled and showed drift to 100% MDCK lineage. Despite being of canine origin, rather than human as was previously thought, MDCK is similarly a distal tubule epithelial cell line. For this reason, previous interpretation and conclusions drawn using these cells here and in Caralt et al., 2015, Uzarski et al., 2015 remain sound, but for purposes of rigor, reproducibility and experimental validation by others we report on this misidentification. This information will also be reported for listing on the International Cell Line Authentication Committee (ICLAC) database (http://iclac.org).

초록

An erratum was issued for Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development.

The human RCTEC/RCTE cell stocks used here and in Caralt et al., 2015, Uzarski et al., 2015 were originally provided by Dr. Loghman-Adham (then at St. Louis University) to co-author Dr. Wandinger-Ness (University of New Mexico) through an MTA in 2001. The SV40 immortalized human RCTEC/RCTE cells were characterized as being of distal tubule cell line as detailed in Loghman-Adham et al., 2003. On the basis of recent short tandem repeat (STR) DNA sequencing (Performed by IDEXX BioResearch) of the earliest passages of the RCTEC/RCTE cell stocks it became evident the cells were of mixed lineage. Further analyses of PCR products using QIAxcel capillary electrophoresis demonstrated the presence of a canine product. The product was sequenced and established to be of canine origin. A set of canine specific STR markers were compared to the sample and showed that the sample had a genetic profile with 92% identity to the MDCK cell line.  Later cell stocks that were used in the present publication were STR profiled and showed drift to 100% MDCK lineage. Despite being of canine origin, rather than human as was previously thought, MDCK is similarly a distal tubule epithelial cell line. For this reason, previous interpretation and conclusions drawn using these cells here and in Caralt et al., 2015, Uzarski et al., 2015 remain sound, but for purposes of rigor, reproducibility and experimental validation by others we report on this misidentification. This information will also be reported for listing on the International Cell Line Authentication Committee (ICLAC) database (http://iclac.org).

프로토콜

신장 조직 개발을 위한 상피 세포 재집락화 및 설치류 세포외 기질 스캐폴드 준비에 대한 정오표가 발행되었습니다.

본 연구 및 Caralt 연구에서 사용된 인간 RCTEC/RCTE 세포주 등, 2015, Uzarski 등, 2015년 당시 St. Louis University 소속이었던 Loghman-Adham 박사가 2001년 MTA를 통해 공동 저자인 Wandinger-Ness 박사(University of New Mexico)에게 처음 제공하였습니다. SV40 불멸화 인간 RCTEC/RCTE 세포는 다음의 상세 내용과 같이 원위세뇨관 세포주인 것으로 특성화되었습니다. Loghman-Adham 등, 2003년, RCTEC/RCTE 세포주 초기 계대의 최근 단기 반복 서열(STR) DNA 시퀀싱(IDEXX BioResearch 수행) 결과, 해당 세포들이 혼합 계통임이 분명해졌습니다. QIAxcel 모세관 전기영동을 이용한 PCR 산물의 추가 분석을 통해 개 유래 산물의 존재가 확인되었습니다. 해당 산물을 시퀀싱한 결과 개 유래임이 입증되었습니다. 일련의 개 특이적 STR 마커를 샘플과 비교한 결과, 해당 샘플은 MDCK 세포주와 92%의 동일성을 갖는 유전적 프로파일을 보였습니다.  본 논문에 사용된 이후의 세포 스톡은 STR 분석을 수행하였으며, 100% MDCK 계통으로의 표류(drift)가 확인되었습니다. MDCK는 이전에 생각했던 인간 유래가 아니라 canine(개) 유래임에도 불구하고, 마찬가지로 원위세뇨관 상피세포주입니다. 이러한 이유로, 본 연구 및 이전 연구에서 이 세포들을 사용하여 도출한 해석과 결론은 카랄트 등, 2015, Uzarski 외 저자들, 2015년의 결과는 여전히 유효하나, 엄격성, 재현성 및 타 연구자에 의한 실험적 검증을 위해 이러한 오동정 사실을 보고합니다. 이 정보는 International Cell Line Authentication Committee (ICLAC) 데이터베이스(http://iclac.org)에 등재하기 위해서도 보고될 예정입니다.