JoVE Journal > Chemistry
Summary
Abstract
Introduction
Protocol
Results
Discussion
Disclosures
Acknowledgements
Materials
References
Chemistry

안 구내 렌즈 응용 프로그램에 대 한 부드러운 Polysiloxane-우 레 탄성 체의 합성

Published: March 08, 2019
doi:
10.3791/58590
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, , , ,
1Reutlingen Research Institute,Reutlingen University2School of Applied Chemistry,Reutlingen University3Fraunhofer-Institute for Interfacial Engineering and Biotechnology IGB4Institute of Interfacial Process Engineering and Plasma Technology IGVP,University of Stuttgart

Summary

Abstract

Introduction

Protocol

Representative Results

Discussion

Disclosures

The authors have nothing to disclose.

Acknowledgements

Materials

Octamethylcyclotetrasiloxane (D4), 97 %ABCR GmbHAB111277presumably impairs fertility, must be degassed before use<br/> <strong>CAS</strong>:&nbsp;556-67-2<br/>
1,3-Bis(3-aminopropyl)-tetramethyldisiloxane, 97%ABCR GmbH110832sensitive to air, must be stored under nitrogen<br/> <strong>CAS</strong>:&nbsp;2469-55-8<br/>
2,4,6,8-Tetramethyl-2,4,6,8-tetraphenylcyclotetrasiloxane&nbsp;Sigma Aldrich40094technical grade<br/> <strong>CAS</strong>:&nbsp;77-63-4<br/>
Tetramethylammonium hydroxide pentahydrateAlfa AesarL09658toxic if swallowed and upon skin contact, strong base, sensitive to air, hygroscopic, store under refrigeration and under nitrogen<br/> <strong>CAS</strong>:&nbsp;10424-65-4<br/>
4,4&cent;-Methylenbis(cyclohexylisocyanate) (H12MDI)Covestro via CSC J&auml;kle Chemie GmbH &amp; Co. KGtoxic if inhaled, skin and eye irritant<br/> <strong>CAS</strong>:&nbsp;5124-30-1<br/>
Tetrahydrofuran (anhydrous) 99.8 %Alfa Aesar44608stabilized with BHT<br/> <strong>CAS</strong>:&nbsp;109-99-9<br/>
Chloroform 99 %Gr&uuml;ssing GmbH Analytica1025125000stabilized with ethanol, presumably carcinogenic, can impair fertility and cause damage to an unborn child<br/> <strong>CAS</strong>:&nbsp;67-66-3<br/>
Chloroform-d, 99.8 %Sigma Aldrich151823<strong>CAS</strong>:&nbsp;865-49-6<br/>
Dulbecco's modified Eagle's medium (DMEM) high glucoseThermo Fisher Scientific Life Technologies GmbH41965-039
Fetal bovine serum (FBS)Thermo Fisher Scientific Life Technologies GmbHA3160801
Trypsin/EDTA, 0.25 % phenol redThermo Fisher Scientific Life Technologies GmbH25200056
Cell Titer Aqueous One Solution cell proliferation assay (MTS)Promega GmbHG3580
HaCaT-cellsCLS Cell Lines Service GmbH300493
BioComFold&nbsp;Morcher GmbHfoldable accommodating intraocular lens
Accommodative 1CUHuman Optics AGfoldable accommodating intraocular lens
CrystaLens&nbsp;Bausch and Lomb Inc.foldable accommodating intraocular lens
Silmer OH-Di10Siltech Corp.Carbinol-terminated Polydimethylsiloxane
Synchrony&nbsp;Visiogen Inc.dual-optic foldable accommodating intraocular lens
Elast-EonAorTech International plcthermoplastic PDMS-PHMO-based polyurethane for medical applications
Pellethane 2363-80ALubrizol Life Sciencesthermoplastic polyether-based polyurethane for medical applications
Zwick universal tensile testing machine model 81565 and software testXpert IIZwick GmbH &amp; Co. KGtensile testing machine
CASYRoche Innovatis AGcell counting system
MultisizerBeckman Coulter Life Sciencescell counting system
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Tags

Polysiloxane-urea ElastomersAmino-terminated PolysiloxanesAccommodating Intraocular LensCataract TherapyPolysiloxane SynthesisPolymerizationMolecular Weight DeterminationSoft MaterialsTransparency
Synthesis of Soft Polysiloxane-urea Elastomers for Intraocular Lens Application

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Riehle, N., Thude, S., Kandelbauer, A., Tovar, G. E. M., Lorenz, G. Synthesis of Soft Polysiloxane-urea Elastomers for Intraocular Lens Application. J. Vis. Exp. (145), e58590, doi:10.3791/58590 (2019).
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