Erratum: A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer

341 ์กฐํšŒ์ˆ˜

โธฑ

2018๋…„ 1์›” 2์ผ

์ด ๋…ผ๋ฌธ์—์„œ

์š”์•ฝ

An erratum was issued for: A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer. There was a typo in step 3.3 of the Protocol.

์ดˆ๋ก

An erratum was issued for: A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer. There was a typo in step 3.3 of the Protocol.

ํ”„๋กœํ† ์ฝœ

An erratum was issued for: A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer. There was a typo in step 3.3 of the Protocol.

Step 3.3 was corrected from:

Add the cell-DNA mixture to the electroporation cuvette and place it in the electroporator chamber. Measure the impedance by pushing the appropriate button on the electroporator and ensure that it is 0.030-0.055 ฮฉ

to:

Add the cell-DNA mixture to the electroporation cuvette and place it in the electroporator chamber. Measure the impedance by pushing the appropriate button on the electroporator and ensure that it is 0.030-0.055 kฮฉ

๊ณต๊ฐœ ์‚ฌํ•ญ

No conflicts of interest declared.

์žฌ์ธ์‡„ ๋ฐ ํ—ˆ๊ฐ€

ํƒœ๊ทธ

Mouse OrganoidsSmall IntestineOrganoid ProtocolMultiple Gene EditingGenome EditingMouse Small IntestineCRISPR Knockout