Dual In Vivo Imaging
Dual In Vivo Imaging - Related Videos
07:33
In vivo Dual Substrate Bioluminescent Imaging
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Cited by 20 •
2011
Herein we describe the methods to construct, visualize, and quantify the bioluminescent reactions of both firefly and renilla luciferase enzymes expressed in metastatic breast cancer cells during their growth and metastasis in vivo.
11:06
In vivo Neuronal Calcium Imaging in C. elegans
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Cited by 20 •
2013
With its small transparent body, well-documented neuroanatomy and a host of amenable genetic techniques and reagents, C. elegans makes an ideal model organism for in vivo neuronal imaging using relatively simple, low-cost techniques. Here we describe single neuron imaging within intact adult animals using genetically encoded fluorescent calcium indicators.
03:16
Dual Fluorescence Imaging to Identify Differentiated Oligodendrocytes
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2025
Source: Schott, J. T., et al. Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning. J. Vis. Exp. (2016). This video demonstrates the use of dual fluorescence imaging to evaluate the differentiation of oligodendrocytes from precursor cells (OPCs) isolated from a rat pup brain. The process includes fixing the cells, labeling specific markers with primary and fluorophore-conjugated secondary antibodies, and...
11:35
Dual-mode Imaging of Cutaneous Tissue Oxygenation and Vascular Function
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Cited by 16 •
2010
A dual-mode imaging system was developed for non-contact assessment of cutaneous tissue oxygenation and vascular function.
09:29
Fabrication and Implantation of Miniature Dual-element Strain Gages for Measuring In Vivo Gastrointestinal Contractions in Rodents.
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Cited by 5 •
2014
The in vivo measurement of smooth muscle contractions along the gastrointestinal tract of laboratory animals remains a powerful, though underutilized, technique. Flexible, dual element strain gages are not commercially available and require fabrication. This protocol describes the construction of reliable, inexpensive strain gages for acute or chronic implantation in rodents.
FAQs
Spatial co-registration aligns measurements from two imaging approaches so that signals can be interpreted at corresponding locations within the organism. This alignment helps relate anatomical features to molecular, functional, or cellular readouts rather than viewing each dataset independently. In bioengineering studies, the combined view can clarify how tissue structure relates to function or how a biomaterial performs over time.
Anatomical information shows where a structure or change occurs, whereas molecular, functional, or cellular measurements provide complementary information about biological activity or response. Pairing these readouts can reveal relationships that one measurement alone may not show. This is particularly relevant when evaluating tissue engineering, drug delivery, disease progression, or responses associated with biomaterials.
Simultaneous acquisition can associate complementary signals during the same observation, while sequential acquisition allows the methods to be used one after another. Both strategies support comparison between imaging readouts, but the timing of acquisition becomes part of how the results are interpreted. The choice therefore depends on how researchers need to relate structural, molecular, functional, or cellular information.
These modalities can provide complementary views rather than identical measurements. Optical imaging, ultrasound, and magnetic resonance imaging may be paired so that one approach contributes information that strengthens or contextualizes the other. The resulting combination is useful when a study needs more than a single type of readout, such as anatomical detail together with molecular, functional, or cellular information.
A study first identifies the biological structure, process, or response to monitor and then selects two complementary imaging approaches. The methods are acquired simultaneously or sequentially, and their measurements are spatially co-registered for comparison. Researchers can then examine relationships between structure and function, assess tissue or biomaterial responses, and follow changes over time without relying only on invasive sampling.
The approach is useful when researchers need longitudinal information about living systems, including tissue-engineering outcomes, drug-delivery behavior, disease progression, or biomaterial performance. Repeated imaging can track structural and functional changes over time while reducing the need for repeated invasive sampling. This makes it valuable for connecting biological responses with engineered materials or interventions during ongoing studies.