Islet Adhesion Technique
Islet Adhesion Technique - Related Videos
09:41
Confocal Imaging of Neuropeptide Y-pHluorin: A Technique to Visualize Insulin Granule Exocytosis in Intact Murine and Human Islets
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Cited by 12 •
2017
We describe a protocol for visualization of insulin exocytosis in intact islets using pHluorin, a pH-sensitive green fluorescent protein. Isolated islets are infected with adenovirus encoding pHluorin coupled to the vesicle cargo neuropeptide Y. This allows for the detection of insulin granule fusion events by confocal microscopy.
05:10
Imaging Calcium Dynamics in Pancreatic Cells: A Technique to Study Real-time Changes in Cytosolic Calcium Concentration in Pancreatic Islet
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2023
This video describes the technique of imaging islet cells in real-time, to study the changes in cytosolic calcium concentration in the minor subpopulations of the pancreatic islet cells. Minor cell subpopulations can be identified using functional responses to marker compounds, such as adrenaline and ghrelin, that induce changes in cytosolic calcium in a specific populations of islet cells. This can provide an insight into the cross talk between the blood glucose and the islet secretory output.
07:21
Pancreatic Islets Isolation through Collagenase Perfusion: An Enzymatic Method to Isolate Pancreatic Islet Cells
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2023
Pancreatic islets, also called the Islets of Langerhans, are a cluster of endocrine cells which produces hormones for glucose regulation and other important biological functions. This protocol explains a method for isolating pancreatic islets by first perfusing collagenase via Ampulla of Vater to digest the exocrine pancreatic tissue and a simplified density gradient centrifugation to purify the islets from mice.
03:33
Grafting Pancreatic Islets: A Method to Implant Pancreatic Islets into the Anterior Chamber of the Mouse Eye for In Vivo Imaging
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2023
This video describes the protocol for grafting human pancreatic islets into the anterior chamber of the mouse’s eye to monitor revascularization of human islet grafts in vivo. It also helps to understand the contribution of recipient versus donor cells in promoting the encapsulation and vascularization of the graft.
03:48
Murine Model of Islet Transplantation: A Surgical Method to Infuse Pancreatic Islets in Portal Vein and Prevention of Postoperative Bleeding
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2025
In this video, we describe a procedure to infuse pancreatic islet cells into the hepatic portal vein, followed by application of gel foam to successfully prevent bleeding. Postoperative bleeding is a common cause of high mortality during islet transplantation.
FAQs
Cell–matrix interactions provide contact between the islet surface and an adhesive or extracellular-matrix-coated substrate. As the islet settles, these interactions promote attachment and limit movement caused by handling or microscopy. Stabilization is important because it improves access to the same three-dimensional cluster over time while helping preserve its native cellular organization for observation and analysis.
Maintaining the three-dimensional arrangement retains the spatial relationships among cells within the endocrine tissue. Those relationships can be examined alongside morphology, differentiation, and maturation rather than being assessed only in isolated cells. The technique therefore supports observations in which tissue organization and interactions between neighboring cells contribute to developmental interpretation.
Attachment reduces the islet's movement across the culture surface, which makes repeated handling, imaging, and morphological analysis more practical. A freely moving preparation can be harder to keep within the field of view or assess consistently. By securing the cluster without removing its three-dimensional architecture, the method improves experimental access while retaining tissue-level structure.
A typical workflow uses a culture surface coated with an adhesive or extracellular-matrix material, followed by placement of pancreatic islets onto that substrate. The islets are allowed to settle so cell–matrix interactions can establish attachment. Once secured, they can be maintained under controlled culture conditions and examined during handling, microscopy, or other analyses.
Attachment facilitates morphological analysis, live-cell observation, and imaging because the three-dimensional clusters remain more stable during examination. Researchers can more readily inspect islet shape and organization, follow cells within an intact cluster, and analyze developmental features under controlled culture conditions. These outcomes make the preparation useful when movement would otherwise interfere with visual or experimental access.
In developmental biology, secured islets provide a model for examining organization, cell differentiation, and maturation within preserved tissue architecture. The preparation also supports investigation of how cellular interactions influence endocrine tissue development. Because the clusters remain accessible for observation in controlled culture, researchers can connect visible structural features with broader developmental processes.