Organoid Viability Assay
Organoid Viability Assay - Related Videos
03:24
ATP-Based Luciferase Viability Assay: A Homogenous Method to Evaluate the Growth-Inhibitory Potential of Test Agents on Tumor Organoids
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2025
In this video, we demonstrate a rapid, single-addition assay to assess cell viability in patient-derived organoids upon treatment with a test agent using intracellular ATP levels as a marker for cellular metabolic activity. The intracellular ATP levels are measured using the luciferase/luciferin reaction, where the emitted light intensity indicates the cellular viability and, in turn, the test agent's growth-inhibitory potency.
12:03
Viability Assays for Cells in Culture
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Cited by 39 •
2014
Therapeutic compounds are often first examined in vitro with viability assays. Blind cell counts by a human observer can be highly sensitive to small changes in cell number but do not assess function. Computerized viability assays, as described here, can assess both structure and function in an objective manner.
03:20
XTT Assay: A Colorimetric Assay to Assess Cell Viability and Mitochondrial Activity
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2023
This article describes the protocol for XTT assay which is a colorimetric assay to assess cell viability and mitochondrial activity.
07:04
Forskolin-induced Swelling in Intestinal Organoids: An In Vitro Assay for Assessing Drug Response in Cystic Fibrosis Patients
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Cited by 121 •
2017
This protocol describes an assay for measuring CFTR function and CFTR modulator responses in cultured tissue from subjects with cystic fibrosis (CF). Biopsy-derived intestinal organoids swell in a cAMP-driven fashion, a response that is defective (or strongly reduced) in CF organoids and can be restored by exposure to CFTR modulators.
03:55
Agarose Spin Down Assay: A Technique to Embed Organoids for Histological Analysis
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2023
In this video, we perform a spin-down assay to embed 3D prostate cancer organoids in agarose. These embedded organoids can be used for further histopathological analysis to study cancer growth and development.
FAQs
Membrane-integrity indicators reveal whether cells have maintained the boundary that separates them from their surroundings, whereas metabolic indicators reflect cellular biochemical activity. These readouts describe different aspects of cell status, so comparing them can help distinguish broadly viable organoids from samples showing cellular damage or reduced activity under the same culture conditions.
Changes in organoid size, shape, or organization do not necessarily indicate healthy maturation. A viability assay adds cellular-status information to structural observations, helping determine whether a developmental change reflects growth or differentiation rather than cellular stress or loss of viability. This distinction is especially important when comparing developmental models or culture conditions.
Culture conditions, genetic changes, and environmental changes can all alter the viability signal measured from an organoid. Because the assay is used under defined conditions, researchers can compare measurements across these variables and identify whether a change in organoid development is associated with altered cellular survival or activity rather than morphology alone.
Both fluorescence and colorimetric measurements can report viability-related indicators, but they provide different measurement formats rather than identical biological information. The choice depends on which indicator is being assessed and how the experiment is designed to compare samples. Either approach can support evaluation of organoid responses when interpreted alongside developmental and structural observations.
A general workflow begins by maintaining organoids under defined culture conditions, applying an indicator of membrane integrity, metabolic activity, or both, and measuring the resulting fluorescence or colorimetric signal. Researchers then compare the measurements across organoids or conditions to evaluate cellular status and relate the result to formation, growth, or differentiation.
Viability measurements provide an outcome for testing whether defined culture conditions support healthy organoid development. By comparing signals across conditions, researchers can identify settings associated with stronger cellular viability or activity and distinguish them from settings that produce stress or loss of viability. This information supports refinement of developmental culture systems.
In developmental biology, these assays help evaluate organoid formation, growth, and differentiation while accounting for cellular status. They also enable comparisons between models exposed to genetic or environmental changes. The resulting viability information helps researchers interpret developmental phenotypes more accurately and determine whether observed differences reflect maturation, stress, or reduced survival.