Pdms Nano Wells
Pdms Nano Wells - Related Videos
09:41
Stretching Micropatterned Cells on a PDMS Membrane
0 Views •
Cited by 6 •
2014
This manuscript presents a technique to apply or release forces on adherent cells or tissues using unidirectional stretching.
14:48
PDMS Device Fabrication and Surface Modification
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Cited by 5 •
2007
04:45
Non-plasma Bonding of PDMS for Inexpensive Fabrication of Microfluidic Devices
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Cited by 19 •
2007
In this video we demonstrate how to use the neuron microfluidic device without plasma bonding.
11:52
Microbubble Fabrication of Concave-porosity PDMS Beads
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Cited by 2 •
2015
Procedures used to generate microstructured concave-porosity polydimethylsiloxane beads are presented. Effects of electrolyte concentration and identity within the aqueous phase are particularly emphasized.
08:58
Double Emulsion Generation Using a Polydimethylsiloxane (PDMS) Co-axial Flow Focus Device
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Cited by 8 •
2015
Microfluidic double emulsions generation typically involves devices with patterned wettability or custom-fabricated glass components. Here we describe the fabrication and testing of an all polydimethylsiloxane (PDMS) double emulsion generator that does not require surface treatment or complicated fabrication processes, and is capable of producing double emulsions down to 14 µm.
FAQs
Physical separation assigns cells, pathogens, or assay reagents to defined nanoscale volumes, reducing mixing between neighboring reactions. This allows a measured signal to be associated with a particular cell or small cell population rather than averaged across a bulk sample. In immunology and infection studies, that separation supports more precise assessment of cell-specific secretion, interactions, and infection outcomes.
The optically clear PDMS material permits microscopy while cells, pathogens, and assay signals remain confined in the wells. Researchers can therefore connect visual information about cell identity or interactions with measurements of secreted signals. This combined observation is valuable when functional behavior differs among individual immune cells or when infection-related responses must be interpreted at cellular resolution.
Because measurements can be linked to individual cells or small groups, the platform distinguishes strong, weak, and undetectable responses that may be obscured in population-level averages. This is particularly useful for identifying rare antigen responses or uncommon cytokine-secreting cells. The resulting measurements describe variation within an immune population, rather than only its overall mean behavior.
A general workflow places selected cells, pathogens, or assay reagents into the defined wells, maintains their physical separation, and examines the compartments by microscopy or signal measurement. Researchers then associate observed secretion or interaction-dependent activity with the relevant cell or small population. The workflow is adaptable to assays focused on immune activation, host-pathogen interaction, or infection outcomes.
Applications include quantifying antigen responses, cytokine secretion, immune-cell behavior, microbial invasion, host-pathogen interactions, and infection outcomes. The same compartmentalized format can examine whether responses vary among cells and can connect those differences with visible cellular or interaction-dependent signals. This makes the approach relevant to both fundamental studies of immune function and therapeutic screening.
These platforms can associate cell identity with functional measurements, allowing researchers to detect rare responses and characterize heterogeneity within a sample. Instead of reporting only a combined signal from many cells, an experiment can indicate which individual cells or small populations secreted factors or displayed interaction-dependent activity. That resolution improves interpretation of immune activation and infection-related variability.