Herbal Medicine Preparation
Herbal Medicine Preparation - Related Videos
08:07
Preparation of Herbal Medicine: Er-Xian Decoction and Er-Xian-containing Serum for In Vivo and In Vitro Experiments
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Cited by 6 •
2017
Here, we present the preparation of an er-xian decoction (EXD) in four steps—soaking, decoction, filtration, and concentration—and demonstrate the administration of a prepared EXD-containing serum to rats. These methods are applicable to the in vivo and in vitro study of herbal decoctions such as traditional Chinese medicines.
07:34
Use of Animal Model of Sepsis to Evaluate Novel Herbal Therapies
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Cited by 13 •
2012
Sepsis refers to a systemic inflammatory response syndrome resulting from a microbial infection, and can be simulated by a surgical technique termed cecal ligation and puncture (CLP). Here we describe a method to use CLP-induced animal model to screen medicinal herbs for therapeutic agents.
06:19
Chinese Herbal Retention Enema for the Treatment of Ulcerative Colitis
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2025
This article demonstrates a Chinese herbal retention enema method for treating ulcerative colitis, which can effectively improve patients' clinical symptoms and quality of life.
06:14
Automating Tumor Implantation in Zebrafish Larvae for Cancer Research and Medicine
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2025
Here, we present a detailed protocol for automated tumor xenograft implantation into zebrafish larvae using a state-of-the-art robotic injection system. Furthermore, we present injection videos and validation data demonstrating the system's successful performance.
16:19
Localized RNAi and Ectopic Gene Expression in the Medicinal Leech
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Cited by 5 •
2008
In this video, we show a procedure for an accurate biolistic delivery of reagents into live tissue with a novel miniature gene gun. We are knocking down the expression of the axon guidance molecule Netrin in leech embryos by delivering molecules of dsRNA into the ventral body wall and ganglia of single segments.
FAQs
Drying and grinding influence how consistently plant material can be processed. Drying is used to help preserve desired constituents, while grinding prepares the material for subsequent extraction or formulation. Because processing can affect the constituents ultimately delivered, these steps matter when producing teas, tinctures, powders, or capsules for evaluation.
Solubility and stability guide the choice of preparation method. Water-based infusion or decoction is appropriate when heat and water can release the desired constituents, whereas maceration or another solvent extraction may be selected when different solvent conditions are needed. Stability also matters because unsuitable conditions may reduce the constituents intended for study or use.
Quality control begins with confirming plant identity and continues through dose, contamination, and storage checks. These factors determine whether different batches provide comparable exposure and whether a preparation remains suitable for evaluation. In medical research or clinical use, documenting such controls helps distinguish effects of the botanical material from variation caused by inconsistent dosing or poor storage.
Infusion, decoction, and maceration should not be treated as interchangeable. Their differing combinations of heat, water, alcohol, or other solvents can change which constituents are released and how stable they remain. Comparing the methods helps investigators select conditions that fit the plant material and the intended therapeutic product.
A controlled workflow starts with identified plant material, followed by a selected process such as drying, grinding, infusion, decoction, maceration, or solvent extraction. The resulting liquid or solid is then filtered or formulated as appropriate, with dose, contamination, and storage considered before use. This sequence supports repeatable preparation for research or clinical evaluation.
Laboratory researchers can use prepared botanical products to investigate constituents and evaluate how preparation conditions affect the material obtained. Clinical use requires attention to dose and consistency, while quality assurance focuses on identity, contamination, and storage. Together, these applications connect traditional remedies with controlled evaluation and development of standardized botanical medicines.