Murine Cancer Model
Murine Cancer Model - Related Videos
06:48
An Orthotopic Murine Model of Human Prostate Cancer Metastasis
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Cited by 30 •
2013
This orthotopic model of human prostate cancer allows for quantification of tumor size, circulating tumor cells, and formation of distinct metastasis to the lung. As cells must escape the primary organ, enter the blood stream, and implant into a secondary site, this model effectively recapitulates the scenario in humans.
03:19
Generating PDOX Mouse Model of Colorectal Cancer: Studying Progression of Colorectal Cancer in a Murine Model
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2023
This video describes the protocol for generating a patient-derived orthotopic xenograft colorectal cancer mouse model to study the progression of colorectal cancer cells in non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice. This murine model helps in understanding primary tumor growth and metastases pattern.
09:07
An Orthotopic Model of Murine Bladder Cancer
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Cited by 24 •
2011
Bladder tumors are established in female mice in a minimally invasive fashion through catheterization, local cauterization, and subsequent adhesion of carcinoma cells to the burn sites.
07:01
Pre-clinical Orthotopic Murine Model of Human Prostate Cancer
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Cited by 17 •
2016
Prostate cancer is the second most common cause of cancer-related deaths in the United States. An orthotopic cancer model provides a useful approach to understand the biology of prostate cancer and to evaluate the efficacy of therapeutic regimens. This protocol describes detailed steps necessary to establish an orthotopic prostate cancer mouse model.
04:35
Murine Ileal Resection Model: Studying Ileectomy-Induced Bile Metabolism in Murine Model
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2023
This video describes the detailed protocol for intestinal resection in a murine model. This technique provides insights into ileectomy-induced bile malabsorption, overaccumulation, and toxicity in the mouse intestine.
FAQs
Implanted models introduce tumor cells, transplantation places tumors in immunodeficient hosts, and engineered models activate oncogenes or disable tumor suppressors within the mouse. These approaches represent different routes to generating disease and therefore support different experimental questions. Comparing them helps researchers connect tumor development with genetic changes, host conditions, and treatment response without treating all mouse models as biologically interchangeable.
An intact immune system allows researchers to examine interactions between tumor cells and immune defenses, rather than observing tumor behavior in isolation. Syngeneic models preserve this context, making them useful for studying how immunity contributes to tumor progression and treatment response. By contrast, immunodeficient hosts are suited to transplantation approaches when the absence of normal immune activity is part of the experimental design.
Murine cancer models can be used to investigate the tumor microenvironment, the surrounding biological setting that influences tumor behavior. They also support analysis of metastasis and therapeutic resistance, linking local tumor conditions with disease progression and treatment outcomes. This broader view helps researchers study cancer as an interaction between tumor biology and its surrounding host environment.
Model selection should follow the biological question and the outcome researchers need to measure. A model generated through implantation, transplantation, or engineered mutations may be chosen to examine tumor development, progression, immune interactions, metastasis, or treatment response. Matching the model to the intended question strengthens interpretation because each approach reproduces different aspects of human cancer biology.
At a high level, researchers generate a tumor using an appropriate model approach, apply an anticancer treatment, and assess measurable changes in disease or response. The resulting observations can connect molecular mechanisms with treatment outcomes. This preclinical framework helps investigators examine therapeutic activity and resistance before using findings to guide further medicine research.
These models help connect molecular mechanisms with measurable differences in tumor behavior and treatment response. By examining how particular tumor features, immune contexts, or engineered changes relate to therapeutic outcomes, researchers can investigate why treatments work differently across disease settings. The findings may guide personalized medicine studies by linking biological characteristics with potential treatment strategies.