Micro-pump Injection

Micro-pump injection is a controlled drug-delivery method that uses a small mechanical pump to administer precise volumes of fluid over a defined period. In neuroscience, the pump connects to a catheter or cannula, often positioned stereotaxically in a specific brain region or near the spinal cord, while programmed flow rates regulate infusion and limit dosing variability. This approach supports localized delivery of pharmacological agents, neurotransmitter modulators, or other experimental solutions with less repeated handling than manual injections. By improving temporal and spatial control, micro-pump injection helps researchers investigate neural circuits, evaluate drug effects, and model neurological conditions.

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Research

JoVE Journal - Engineering
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Prescribed 3-D Direct Writing of Suspended Micron/Sub-micron Scale Fiber Structures via a Robotic Dispensing System

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Cited by 6 •

2015

Here, we present a protocol to fabricate freely-suspended, micron/sub-micron scale polymer fibers and “web-like” structures generated via automated direct writing procedure by means of a 3-axis dispensing system.

Research

JoVE Journal - Developmental Biology

Subcutaneous Neurotrophin 4 Infusion Using Osmotic Pumps or Direct Muscular Injection Enhances Aging Rat Laryngeal Muscles

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Cited by 7 •

2017

Here, we present a protocol to describe the use of neurotrophin 4 (NTF4) systemically and directly to remodel rat aging laryngeal muscles.

Education

JoVE Core - Cell Biology

ATP Driven Pumps III: V-type Pumps

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2023

V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient. The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...

ATP Driven Pumps II: P-type Pumps

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2023

The P-type pumps are a large family of integral membrane transporter ATPases. They are divided into five major types based on substrate specificity, from I to V. A typical P-type pump has three cytosolic domains: nucleotide-binding (N), phosphorylation (P), and activator (A) domains. These domains are connected to the membrane-spanning helices by short amino acid segments. ATP hydrolysis and covalent phosphoenzyme intermediate formation are crucial parts of the catalytic cycle. At the highly...

Mechanical Micronization of Lipoaspirates for Regenerative Therapy

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Cited by 18 •

2019

Here, we present a protocol to obtain stromal vascular fraction from adipose tissue through a series of mechanical processes, which include emulsification and multiple centrifugations.

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