혈액 채취 II
View the full transcript and gain access to JoVE Science Education videos
Q1: What are the advantages of facial and submandibular vein blood collection in mice?
Facial and submandibular vein collection methods do not require anesthesia, making them suitable when anesthetics may confound blood results or other data. These less invasive procedures are methods-of-choice for studies where anesthetic interference is a concern. Both techniques allow researchers to collect blood while minimizing experimental variables.
Q2: How do you properly restrain a mouse for facial vein blood collection?
Use the scruffing technique to restrain the animal, minimizing side-to-side head movement for accurate venipuncture. Avoid gripping too tightly, as excessive pressure restricts blood flow and reduces collection volume. Proper restraint ensures safe and efficient blood collection from the facial vein running across the cheek.
Q3: What is the correct needle insertion depth for submandibular vein puncture?
Do not insert the needle beyond the bevel tip, as deeper insertion may cause trauma to muscles, nerves, and other vessels in the head, neck, and oral cavity. Proper depth control prevents complications while allowing successful blood collection from the submandibular vein.
Q4: How does the saphenous vein collection method differ from femoral vein collection?
Saphenous vein collection yields 10-150 microliters and may contain tissue products, with variable sample quality. Femoral vein collection produces larger volumes more easily and requires two people for conscious animals. Femoral vein puncture should occur near the knee to allow future anterior sampling, whereas saphenous sampling is limited to four samples per leg in 24 hours.
Q5: What equipment is needed for saphenous vein blood collection?
Required equipment includes a restraining device, tourniquet made from a syringe and suture, triple antibiotic ointment, hair clipper, 22-gauge needle, and hematocrit tube. The tourniquet is applied cranially to engorge the saphenous vessel, making it visible and accessible for safe venipuncture and blood collection.
Q6: Why is positioning the animal's head lower than the heart important during blood collection?
Positioning the head lower than the heart assists blood flow by using gravity to facilitate collection. This positioning helps ensure adequate blood volume is obtained and reduces collection time. After collection, apply pressure to the puncture site to achieve hemostasis and prevent excessive blood loss.
Q7: How frequently can the same facial vein site be used for serial blood collection?
Serial samples can be taken by alternating sides of the face, with neither side used more often than every 5-7 days. This interval allows the puncture site to heal and prevents tissue damage. Researchers must also adhere to maximum survival blood collection volumes outlined in Blood Withdrawal I guidelines.