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An ex-ovo Chicken Embryo Culture System Suitable for Imaging and Microsurgery Applications
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An ex-ovo Chicken Embryo Culture System Suitable for Imaging and Microsurgery Applications

An ex-ovo Chicken Embryo Culture System Suitable for Imaging and Microsurgery Applications

DOI:
10.3791/2154-v

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10:59 min

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October 23, 2010

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, , ,
1Department of Biomedical Engineering,Cornell University, 2Current Address: Mechanical Engineering Department,Dogus University

Chapters

  • 00:00Title
  • 00:21Subscription Locl
  • 00:46Introduction
  • 01:37Preparing the Hammocks
  • 02:53 Ex ovo Culturing of the Chicken Embryos
  • 05:26Microinjection into the Vasculature
  • 08:34Left Atrial Ligation
  • 09:53Representative Results
  • 10:30Conclusion

Summary

자동 번역

자동 번역

In this article, we present a simple methodology to enable long-term ex-ovo avian embryo culture. This technique is ideal for longitudinal experimentation requiring complete optical accessibility and/or sterile transportation in avian embryos.

Tags

Ex-ovo Chicken Embryo Culture SystemImagingMicrosurgery ApplicationsGenetic FactorsMicroenvironmental FactorsEmbryonic DevelopmentTherapeutic StrategiesImaging TechnologyBody Plan OrganizationEmbryonic MorphogenesisChicken EmbryosVertebrate Animal Model SystemCulture And Surgical ManipulationFilter Paper RingsOptical AccessCell PatterningFate StudiesCardiac MorphogenesisEggshell Window TechniqueHigh Resolution ImagingUltrasonographyShell-less Culture SystemEnvironmental Chamber

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