마우스에 작은 창자 이식

14.8K 조회수

2회 인용됨

36:30 분

2007년 8월 20일

2007년 8월 20일

14.8K 조회수

Fengchun Liu¹ , Sang-Mo Kang¹

¹Department of Surgery, University of California, San Francisco - UCSF

마우스 작은 창자 이식 모델은 면역 거부하고 화면의 새로운 immunosuppressive 약물 mechanismes을 연구하는 중요한 도구로 인식되었습니다. 그러나이 모델은 관련된 기술이 매우 기술적으로 도전하기 때문에 사용하는 제한됩니다. 이제 우리는 수정 기법을 소개합니다.

더 많은 동영상 탐색

마우스 모델

외과적 수술 기법

면역억제제

거부반응 연구

문맥 문합술

고정 봉합사

Chapters in this video

0:03

Title

0:16

Introduction

1:18

Harvesting Small Bowel from Donor Mouse

1:20

1. performing Tail Pinch to Ensure Mouse is Anesthetized

2:46

2. clearing away tissue to allow ligation of small bowel

4:21

3. cleaning out small bowel contents before ligating ileum

4:50

4. ligating ileum

5:20

5. exposing and ligating mesenteric vessels

6:18

6. exposing mesenteric artery which will be cut when small bowel is

6:42

7. exposing portal vein

7:28

8. ligating branch of portal vein

7:58

9. exposing other side of mesenteric artery

8:09

10. locating inferior vena cava

8:15

11. injecting heparin into the inferior vena cava

8:36

12. cutting mesenteric artery

8:54

13.clearing away tissue from mesentaric artery

9:23

14. cutting jejunal end of ligated small bowel

9:37

15. cutting away small bowel mesenteric tissue

10:27

16. cutting away mesenteric tissue until the portal vein is reached

10:56

17. cutting ileal end of ligated small bowel

11:19

18. cutting away mesenteric tissue

11:34

19. cutting portal vein

11:57

20. harvesting donor small bowel

12:09

Preparing Recipient Mouse for Small Bowel Transplantation

13:20

1. exposing inferior vena cava and aorta

13:29

2. clearing away tissue in order to apply ligatures

14:35

3. applying ligatures to inferior vena cava and inferior aorta

15:03

Connecting Donor Small Bowel Circulation to Recipient Circulation

15:15

1. placing donor small bowel in recipient mouse

15:56

puncturing inferior vena cava for veinotomy

16:17

3. performing veinotomy on inferior vena cava

16:31

4. repeat procedure for aorta

17:00

5. washing away blood from vessels with saline

17:15

6. suturing donor mesenteric artery to recipient aorta

19:59

7. suturing donor portal vein to recipient inferior vena cava

22:39

8. final suture for anastamosis of donor portal vein to recipient i

23:01

9. flipping donor small bowel in order to finish suture of mesenter

23:35

10. suturing donor mesenteric artery to other side of aorta

24:53

11. final suture for anastamosis of donor mesenteric artery to reci

25:56

12. releasing ligatures to open circulation to donor small bowel

26:47

Connecting Donor Small Bowel to Recipient Small Bowel

27:20

1. clamping ileal end of donor small bowel

27:39

2. cutting recipient jejunum

27:50

3. cutting ileal end of donor small bowel

28:04

4. suturing one side of donor ileum to recipient jejunum

29:10

5. flipping small bowel clamps in order to suture other side of ana

30:20

6. removing clamps

31:14

7. creating stoma for jejunal end of donor small bowel

32:02

8. suturing jejunal end of donor small bowel to stoma

32:40

9. cutting ligature to jejunal end of donor small bowel

33:14

Sewing up Abdominal Cavity

35:32

Conclusion