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Agrobacterium-gemedieerde virus-geïnduceerde gen silencing Assay In Cotton

Article DOI: 10.3791/2938-v • 10:18 min • August 20th, 2011
August 20th, 2011 •
1, 1, 2, 1
1Department of Biochemistry and Biophysics, Institute of Plant Genomics and Biotechnology, Texas A&M University, 2Department of Plant Pathology and Microbiology, Institute of Plant Genomics and Biotechnology, Texas A&M University

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We presenteren de gedetailleerde protocol voor Agrobacterium-gemedieerde virus-geïnduceerde gen silencing (VIGS) test in katoen. De tabak rammelaar virus (TRV)-afgeleide VIGS vectoren werden ingezet om RNA-silencing van katoen GrCLA1, Cloroplastos alterados 1 gen induceren. De albino fenotype veroorzaakt door zwijgen op te leggen GrCLA1 ​​werd waargenomen bij de zaailing fase binnen 2 weken na inoculatie.

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Plant Biology Agrobacterium Katoen Functional Genomics virus-geïnduceerde gen silencing
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