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Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR

Article DOI: 10.3791/3232-v • 15:17 min • July 22nd, 2012
July 22nd, 2012 •
1, 1, 1,2, 3, 3, 1, 1, 2, 1, 1,2,4
1Laboratorio de Investigación y Desarrollo (LID), Universidad Peruana Cayetano Heredia, 2Bloomberg School of Public Health, Johns Hopkins University, 3Laboratorio de Diagnostico Molecular, Facultad de Medicina, University of Concepcion,Chile, 4University of California San Diego School of Medicine

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A One-Step RT-PCR assay for detection and genogroup identification of Norovirus isolates from children’s stools, that utilizes primers and TaqMan probes specific to the open reading frame 1 (ORF1)-ORF2 junction region, the most conserved region of the Norovirus genome is described. A non-commercial, cost-effective RNA extraction method is detailed.

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Noroviruses Genogrouping Taqman One-step RT-PCR Acute Gastroenteritis RNA Viruses Genetic Diversity New Strains Genogroups GI GII Genotypes Subtypes Diagnosis Treatment RNA Extraction QIAmp Viral RNA Commercial Kit Silica Gel Membrane Buffers Microspin Diarrhea Pathogenesis
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