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Bioengineering

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Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy

Article DOI: 10.3791/3248-v • 10:25 min • December 9th, 2011
December 9th, 2011 •
1, 2, 3, 1, 1, 1, 4, 1, 5
1Department of Computer Science and Engineering, Texas A&M University, 2Beckman Institute for Advanced Science and Technology, University of Illinois, 3Department of Electrical and Computer Engineering, Kettering University, 43Scan, 5Department of Veterinary Integrative Biosciences, Texas A&M University

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요약
Automatic Translation

The full process from brain specimen preparation to serial sectioning imaging using the Knife-Edge Scanning Microscope, to data visualization and analysis is described. This technique is currently used to acquire mouse brain data, but it is applicable to other organs, other species.

Tags

Specimen Preparation Imaging Analysis Protocols Knife-edge Scanning Microscopy High-throughput Microscopy High-resolution Microscopy 3D Microscopy Neuroanatomical Data Submicrometer Resolution Whole-brain-scale Data Neuronal Networks Vascular Networks Cell Body Distribution Connectomics Research Microcirculation Research Hemodynamic Research Stereology Research Fixing Staining Embedding KESM Configuration And Setup Sectioning And Imaging With KESM Image Processing Data Preparation Data Visualization Data Analysis
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