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Chapters
- 00:05Title
- 01:20Ethyl Methane Sulfonate (EMS) Treatment
- 04:25Confocal Screening of M2 and M3 Populations
- 05:35Mapping
- 09:10Results: Mutation Mapping using Genechip Arabidopsis ATH1 Genome Array Hybridization and R Software Analysis
- 12:24Conclusion
A fundamental quest in cell biology is to define the mechanisms that underlie the identity of the organelles that make eukaryotic cells. Here we propose a method to identify the genes responsible for the morphological and functional integrity of plant organelles using fluorescence microscopy and next-generation sequencing tools.
Tags
Fluorescence-microscopy ScreeningNext-generation SequencingIdentification Of GenesOrganelle IntegrityArabidopsis SeedlingsRecessive MutationsSubcellular DistributionTagged Fluorescent MarkerSecretory PathwayGenetic StudiesGenome SizeGeneration TimeConservation Of Molecular MechanismsArray GenotypingAlternative Mapping MethodMolecular MarkersProteins Influencing Organelle IntegrityEndoplasmic Reticulum (ER)Plant Cell OrganellesMolecular Basis
