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Journal / Biology / Udarbejdelse af muse embryonale fibroblastceller…
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Biology

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Udarbejdelse af muse embryonale fibroblastceller anvendes til dyrkning af menneskelige embryoner og induceret pluripotente stamceller
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Udarbejdelse af muse embryonale fibroblastceller anvendes til dyrkning af menneskelige embryoner og induceret pluripotente stamceller

Article DOI: 10.3791/3854-v • 09:38 min • June 21st, 2012
June 21st, 2012 •
1, 1, 1
1Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics

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Kvaliteten af ​​muse embryonale fibroblaster (MEF) dikteres af den højre musestamme, såsom CF-1. Pluripotency-støttende MEF'er og konditioneret medium (CM) opnået fra disse skal indeholde optimale koncentrationer af Activin A, Gremlin og Tgfβ1 nødvendige for aktivin / Nodal og FGF veje til at co-operativt opretholde selvfornyelse og pluripotency.

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Stem Cell Biology Molekylær Biologi Developmental Biology muse embryonale fibroblaster (MEF'er) humane embryonale stamceller (hESCs) menneskelige inducerede pluripotente stamceller (hiPSCs) Activin A-konditioneret medium (CM) cellekultur
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