JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:34Egg Preparation
- 02:42Glass Injection Needle Preparation
- 03:25Midbrain Injection
- 04:38Electroporation
- 05:39Incubate and Harvest
- 06:47Results: Embryonic Chick Midbrain In ovo Electroporation Assay
- 08:13Conclusion
To assess the function and the regulation of genes during the development of midbrain dopaminergic neurons, we describe a method that involves in ovo electroporation of plasmid DNA constructs into embryonic chick ventral midbrain dopaminergic neuron progenitors. This technique can be used to achieve efficient expression of genes of interest to study different aspects of midbrain development and dopaminergic neuron differentiation.
