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A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System

Article DOI: 10.3791/50439-v • 13:22 min • September 10th, 2013
September 10th, 2013 •
1, 1, 2, 1, 1,3, 1
1Institute of Toxicology and Genetics, Karlsruhe Institute of Technology - Campus North, 2Institute of Organic Chemistry, Karlsruhe Institute of Technology - Campus North, 3Institute of Organic Chemistry, Karlsruhe Institute of Technology - Campus South

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요약
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We describe the procedure and data analysis of a chemical screening system for glucocorticoid stress hormone signaling using zebrafish larvae: the Glucocorticoid Responsive In vivo Zebrafish Luciferase activitY (GRIZLY) assay. The assay sensitively and specifically detects effects on glucocorticoid signaling by compounds that require metabolization or affect endogenous glucocorticoid production.

Tags

Chemical Screening Procedure Glucocorticoid Signaling Zebrafish Larva Luciferase Reporter System Glucocorticoid Stress Hormones Inflammation Treatment Side Effects Test Systems Novel Compounds Glucocorticoid Signaling Pathway Transgenic Zebrafish Assay GRIZLY Assay Luciferase-based Assay Metabolization Endogenous Glucocorticoid Production Chemical Screens Data Acquisition Normalization And Analysis Quality Control Data Visualization Time-resolved Readout Quantitative Readout Stand-alone Platform High-throughput Screening Workflows Environmental Monitoring Endocrine Disruptors Stress Research
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