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Hurtig Protokol for Udarbejdelse af elektrokompetente

Article DOI: 10.3791/50684-v • 07:11 min • October 8th, 2013
October 8th, 2013 •
1, 2, 2, 1,3
1Department of Biological Sciences, University of Texas Brownsville, 2Department of Medical Microbiology and Immunology, University of Alberta, 3Department of Biomedical Sciences, University of Texas Brownsville

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Elektroporation er en almindeligt anvendt fremgangsmåde til indføring af DNA i bakterier i en proces kendt som transformation. Traditionelle protokoller til fremstilling af elektrokompetente celler er tidskrævende og arbejdskrævende. Denne artikel beskriver en alternativ, hurtig og effektiv metode til fremstilling af elektrokompetente celler i øjeblikket ansat af nogle laboratorier.

Tags

Bioengineering Cell Engineering Gram-negative bakterier Enterobacteriaceae Escherichia, Vibrionaceae Vibrio, Bakterier, Electrocompetence transformationsprotokol elektroporation
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