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Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Article DOI: 10.3791/51446-v • 14:43 min • August 27th, 2014
August 27th, 2014 •
1,2, 1, 1,3, 1,4,5,6, 1,4
1LENS - European Laboratory for Non-linear Spectroscopy, University of Florence, 2Chemistry Research Laboratory, University of Oxford, 3Department of Biology, University of Florence, 4Department of Physics and Astronomy, University of Florence, 5National Institute of Optics-National Research Council, Italy, 6International Center of Computational Neurophotonics

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요약
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Here we describe the instrumentation and methods for detecting single fluorescently-labeled protein molecules interacting with a single DNA molecule suspended between two optically trapped microspheres.

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Single-molecule Manipulation Imaging Study Protein-DNA Interactions Optical Tweezers Single Molecule Fluorescence Detection Solution DNA Extension Tracking Interaction Dynamics Mechanical Parameters DNA Sequence Optical Trapping Nanometer Localization Lactose Repressor (lacI) Atto532 Target Sequences LacI Binding Observation Of Specific Interactions One-dimensional Diffusion Target Search Broadly Applicable Molecular Motors Tension Control Partner Track Polymer
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