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Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay
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Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay

Article DOI: 10.3791/51516-v • 11:49 min • July 28th, 2014
July 28th, 2014 •
1, 1, 1, 1, 1, 1, 1
1Department of Biology, KU Leuven

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요약
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The here described fluorescence-based calcium mobilization assay is a medium-throughput reverse pharmacology screening system for the identification of functionally activating ligand(s) of orphan G protein-coupled receptors (GPCRs).

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G Protein-coupled Receptors Reverse Pharmacology Ligands Cellular Expression System Compound Library Calcium Mobilization Assay Second Messenger Reporter Molecules Calcium Indicator Fluo-4 Receptor Activation
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