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Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration
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Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration

Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration

DOI:
10.3791/51682-v

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10:35 min

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September 06, 2014

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, , , , ,
1Division of Cardiothoracic Surgery,Beth Israel Deaconess Medical Center and Harvard Medical School, 2Department of Anesthesiology, Perioperative and Pain Medicine,Boston Children’s Hospital and Department of Anesthesia, Harvard Medical School

Chapters

  • 00:05Title
  • 01:21Harvesting Mitochondria
  • 04:32The ATP Assay
  • 08:18Mitochondria Characterization from Liver and Skeletal Muscle Biopsy
  • 10:16Conclusion

Summary

자동 번역

자동 번역

A method for rapid isolation of mitochondria from mammalian tissue biopsies is described. Rat liver or skeletal muscle preparations were homogenized with a commercial tissue dissociator and mitochondria were isolated by differential filtration through nylon mesh filters. Mitochondrial isolation time is <30 min compared to 60 - 100 min using alternative methods.

Tags

Mitochondrial IsolationRapid IsolationDifferential FiltrationBiopsyNylon Mesh FiltersRespiration Competent Mitochondria

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