Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination

Robert A. Hill; Jelena Medved; Kiran D. Patel; Akiko Nishiyama

doi:10.3791/51835

Journal

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신경과학

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Organotypic 슬라이스는 문화 희소 돌기 아교 세포 역학 및 수초화을 공부하기

Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination

JoVE 신문
신경과학

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JoVE 신문 신경과학

Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination

Organotypic 슬라이스는 문화 희소 돌기 아교 세포 역학 및 수초화을 공부하기

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18,246 Views

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09:45 min

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August 25, 2014

DOI:

10.3791/51835-v

DOI:

10.3791/51835-v

•

09:45 min

August 25, 2014

•

12 Views

Robert A. Hill^1,3, Jelena Medved¹, Kiran D. Patel¹, Akiko Nishiyama^1,2

¹Department of Physiology and Neurobiology,University of Connecticut, ²Stem Cell Institute,University of Connecticut, ³Department of Neurology,Yale University School of Medicine

Summary

A technique to study NG2 cells and oligodendrocytes using a slice culture system of the forebrain and cerebellum is described. This method allows examination of the dynamics of proliferation and differentiation of cells within the oligodendrocyte lineage where the extracellular environment can be easily manipulated while maintaining tissue cytoarchitecture.