An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, <em>Aedes albopictus</em>

Monica F. Poelchau; Xin Huang; Allison Goff; Julie Reynolds; Peter Armbruster

doi:10.3791/51961

Journal

/

생물학

/

아시아 타이거 모기에 Photoperiodic 휴면의 RNA-서열 분석을위한 실험 및 생물 정보학 프로토콜,<em> Aedes albopictus</em

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, <em>Aedes albopictus</em>

JoVE 신문
생물학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 생물학

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus

아시아 타이거 모기에 Photoperiodic 휴면의 RNA-서열 분석을위한 실험 및 생물 정보학 프로토콜,<em> Aedes albopictus</em

Please note that all translations are automatically generated. Click here for the English version.

13,293 Views

•

12:10 min

•

November 30, 2014

DOI:

10.3791/51961-v

DOI:

10.3791/51961-v

•

12:10 min

November 30, 2014

•

13266 Views

Monica F. Poelchau¹, Xin Huang¹, Allison Goff¹, Julie Reynolds², Peter Armbruster¹

¹Department of Biology,Georgetown University ²Insect Physiology Lab, EEOB,The Ohio State University

Summary

RNA-Seq analyses are becoming increasingly important for identifying the molecular underpinnings of adaptive traits in non-model organisms. Here, a protocol to identify differentially expressed genes between diapause and non-diapause Aedes albopictus mosquitoes is described, from mosquito rearing, to RNA sequencing and bioinformatics analyses of RNA-Seq data.