Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Protein Purification Technique that Allows…
JoVE Journal
Biology

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.

JoVE Journal Biology
Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80
Temporal Quantification of MAPK Induced Expression in Single Yeast Cells
Journal (Biology)
Temporal Quantification of MAPK Induced Expression in Single Yeast Cells
Measurement of Smooth Muscle Function in the Isolated Tissue Bath-applications to Pharmacology Research
Journal (Biology)
Measurement of Smooth Muscle Function in the Isolated Tissue Bath-applications to Pharmacology Research
 

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80

Article DOI: 10.3791/52482-v • 12:28 min • May 3rd, 2015
May 3rd, 2015 •
1, 1, 1
1Genetics Branch, Center for Cancer Research, National Cancer Institute, National Institute of Health

챕터

요약
Automatic Translation

This manuscript describes the detection of sumoylation and ubiquitination of kinetochore proteins, Ndc10 and Ndc80, in the budding yeast Saccharomyces cerevisiae.

Tags

Protein Purification Technique Detection Sumoylation Ubiquitination Budding Yeast Kinetochore Proteins Ndc10 Ndc80 Post-translational Modifications PTMs Phosphorylation Methylation Acetylation Mass Spectrometry Western Blot Analysis Biochemical Approaches Genome Stability Saccharomyces Cerevisiae Polyhistidine-Flag-tagged Smt3 Myc-tagged Ndc10 Myc-tagged Ndc80 Affinity Purification Nickel Beads Anti-Myc Antibody Anti-Ub Antibody Epitope Tagged-protein
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter