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Assessing Bacterial Invasion of Cardiac Cells in Culture and Heart Colonization in Infected Mice Using Listeria monocytogenes
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Chapters
- 00:05Title
- 01:54Invasion Assay
- 04:09Inoculating L. monocytogenes into Mice via Tail Vein Injection and Assessing Bacterial Burden in Liver, Spleen, and Heart
- 07:39Results: L. monocytogenes Infection in Cultured Organ Cells
- 08:47Conclusion
Listeria monocytogenes causes fetal infections in pregnant women and meningitis in susceptible populations. Subpopulations of bacteria can colonize cardiac tissue, causing myocarditis in patients and laboratory animals. Here we present a protocol that describes how to assess L. monocytogenes cardiac cell invasion in vitro and cardiac colonization in infected animals.
