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An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis

Article DOI: 10.3791/52827-v • 08:09 min • September 15th, 2015
September 15th, 2015 •
1, 1
1Department of Biology, Concordia University

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요약
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This protocol is for the extraction and concentration of protein and DNA from microbial biomass collected from seawater, followed by the generation of tryptic peptides suitable for tandem mass spectrometry-based proteomic analysis.

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Aquatic Microbial Metaproteomics Workflow Cells Tryptic Peptides Tandem Mass Spectrometry-based Analysis Metagenomics Metatranscriptomics Metaproteomics Microbial Community Structure Metabolism Functional Potential Expressed In Situ Metabolism Function Protocol Cell Lysis Protein Isolation DNA Isolation Peptide Digestion Peptide Extraction Marine Microbial Cells Cartridge Filter Unit Sterivex Filter Unit RNA Stabilization Solution RNAlater Mass Spectrometry-based Proteomics Studies Identification Of Peptides Identification Of Proteins Peptide Tandem Mass Spectra Database Of Translated Nucleotide Sequences
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