Isolation of Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High Throughput Microplate Respiratory Measurements

Nabil E. Boutagy; Emily Pyne; George W. Rogers; Mostafa Ali; Matthew W. Hulver; Madlyn I. Frisard

doi:10.3791/53217

Journal

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생물학

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높은 처리량 마이크로 호흡 측정을위한 마우스 골격근의 최소 수량에서 미토콘드리아의 분리

Isolation of Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High Throughput Microplate Respiratory Measurements

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JoVE 신문 생물학

Isolation of Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High Throughput Microplate Respiratory Measurements

높은 처리량 마이크로 호흡 측정을위한 마우스 골격근의 최소 수량에서 미토콘드리아의 분리

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12,727 Views

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10:12 min

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November 13, 2015

DOI:

10.3791/53217-v

DOI:

10.3791/53217-v

•

10:12 min

November 13, 2015

•

7 Views

Nabil E. Boutagy^1,2, Emily Pyne¹, George W. Rogers³, Mostafa Ali¹, Matthew W. Hulver^1,2, Madlyn I. Frisard^1,2

¹The Department of Human Nutrition, Foods, and Exercise,Virginia Tech, ²The Metabolic Phenotyping Core,Virginia Tech, ³Seahorse Bioscience

Summary

Here, we present a modification of a previously reported method that allows for the isolation of high quality and purified mitochondria from smaller quantities of mouse skeletal muscle. This procedure results in highly coupled mitochondria that respire with high function during microplate based respirometirc assays.