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A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA

Article DOI: 10.3791/53307-v • 06:54 min • December 26th, 2015
December 26th, 2015 •
*1, *1,2, 1,2, 1, 1, 1, 1,2
1CSIR-Institute of Genomics and Integrative Biology, New Delhi, India, 2Academy of Scientific and Innovative Research (AcSIR), New Delhi, India
* These authors contributed equally

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MicroRNAs play crucial roles in the brain and are potential targets for modeling neuro-degeneration. However, perturbing miRNA levels is challenging due to the short length of miRNA and inaccessibility of the brain tissue. This video presents a method for antagomir design and brain specific delivery using a neuropeptide in mice.

Tags

MiRNA MicroRNA Gene Expression Regulation Neurodevelopment Neuronal Functions Knockdown Stereotactic Injection Brain-specific Delivery Antagomirs Locked Nucleotide Modifications (LNA) Cell-penetrating Peptide Rabies Virus Glycoprotein (RVG) Tail Vein Injection Neurodegenerative Diseases Neuronal Cell Death Non-covalent Complexes Apoptotic Cell Death Behavioural Defects
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