Measuring Physiological Responses of Drosophila Sensory Neurons to Lipid Pheromones Using Live Calcium Imaging

Shruti Shankar; Meredith E.K. Calvert; Joanne Y. Yew

doi:10.3791/53392

Journal

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면역학 및 감염병학

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의 생리적 반응을 측정 초파리 라이브 칼슘 이미징을 사용하여 지질 페로몬에 감각 뉴런

Measuring Physiological Responses of <em>Drosophila</em> Sensory Neurons to Lipid Pheromones Using Live Calcium Imaging

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면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Measuring Physiological Responses of Drosophila Sensory Neurons to Lipid Pheromones Using Live Calcium Imaging

의 생리적 반응을 측정 초파리 라이브 칼슘 이미징을 사용하여 지질 페로몬에 감각 뉴런

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8,570 Views

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07:25 min

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April 29, 2016

DOI:

10.3791/53392-v

DOI:

10.3791/53392-v

•

07:25 min

April 29, 2016

•

8535 Views

Shruti Shankar^1,2, Meredith E.K. Calvert^3,4, Joanne Y. Yew^1,2,5

¹Temasek Life Sciences Laboratory ²Department of Biological Science,National University of Singapore ³Bioimaging and Biocomputing Facility,Temasek Life Sciences Laboratory ⁴Histology and Light Microscopy Core,Gladstone Institutes ⁵Pacific Biosciences Research Center,University of Hawai’i at Mānoa

Summary

The forelegs and proboscis of Drosophila contain a rich repertoire of gustatory sensory neurons. Here, we present a method using calcium imaging to measure physiological responses from sensory neurons in the foreleg and proboscis of live flies upon exogenous application of a gustatory pheromone.