A Behavioral Assay for Mechanosensation of MARCM-based Clones in <em>Drosophila melanogaster</em>

Timothy P. Murphy; Dan D. Luu; Jessica A. DeSimone; Thomas C. O'Brien; Christopher J. Lally; Jillian J. Lindblad; Sarah M. Webster

doi:10.3791/53537

Journal

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신경과학

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의 Mechanosensation에 대한 행동 분석 클론의 MARCM은 기반<em> 초파리</em

A Behavioral Assay for Mechanosensation of MARCM-based Clones in <em>Drosophila melanogaster</em>

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A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster

의 Mechanosensation에 대한 행동 분석 클론의 MARCM은 기반<em> 초파리</em

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10,209 Views

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05:48 min

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December 30, 2015

DOI:

10.3791/53537-v

DOI:

10.3791/53537-v

•

05:48 min

December 30, 2015

•

10201 Views

Timothy P. Murphy^1,2, Dan D. Luu¹, Jessica A. DeSimone^1,3, Thomas C. O’Brien^1,4, Christopher J. Lally^1,5, Jillian J. Lindblad^1,6, Sarah M. Webster¹

¹Department of Biology,College of the Holy Cross ²School of Medicine,Georgetown University ³Department of Biochemistry,Giesel School of Medicine, Dartmouth College ⁴School of Medicine,Tufts University ⁵Transgenomic Inc.⁶Department of Molecular, Cell and Cancer Biology,UMass Medical School

Summary

In order to identify novel mutations affecting mechanosensation, we designed an assay that measures the behavioral response to tactile stimulation of fly bristles in mutant clones generated by the MARCM method. The combination of techniques allows for the identification of mechanosensitive mutations that would otherwise be lethal.