Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Chemistry / CN-GELFrEE - Clear Native Gel-eluted Liquid…
JoVE Journal
Chemistry

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.

JoVE Journal Chemistry
CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis
TiO2-coated Hollow Glass Microspheres with Superhydrophobic and High IR-reflective Properties Synthesized by a Soft-chemistry Method
Journal (Chemistry)
TiO2-coated Hollow Glass Microspheres with Superhydrophobic and High IR-reflective Properties Synthesized by a Soft-chemistry Method
Quality Check (QC) of the TCPP Beads by Flow Cytometry (Video) | JoVE
Journal (Chemistry)
Quality Check (QC) of the TCPP Beads by Flow Cytometry (Video) | JoVE
 

CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis

Article DOI: 10.3791/53597-v • 11:38 min • February 29th, 2016
February 29th, 2016 •
*1,2, *1, 1, 1,3, 1, 1, 3, 2, 1, 1
1Departments of Chemistry and Molecular Biosciences, Chemistry of Life Processes Institute, Proteomics Center of Excellence, Robert H. Lurie Comprehensive Cancer Center, Northwestern University, 2Institute of Chemistry, Proteomics Unit, Federal University of Rio de Janeiro, 3Department of Cell Biology, Brazilian Center for Protein Research, Laboratory of Biochemistry and Protein Chemistry, University of Brasilia
* These authors contributed equally

챕터

요약
Automatic Translation

This protocol describes how to prepare and perform clear native gel-eluted liquid fraction entrapment electrophoresis (CN-GELFrEE), a native separation technique for non-covalent biomolecular assemblies and proteins from heterogeneous samples that is compatible with various downstream protein analysis techniques.

Tags

CN-GELFrEE Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis Separations Technique Native Samples Non-covalent Macromolecular Interactions Protein Complexes Recovery And Resolution Complex Composition Stoichiometry High Recovery Low Sample Dilution Casting System Serological Pipette Hot Blade Support Stand Tubing Flow Control Valve Gradient Mixer Magnetic Stirrer Stir Bars Leaks De-ionized Water Tape Layers Glass Tube
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter