Isolation and Characterization of Single Cells from Zebrafish Embryos

Leigh Ann Samsa; Nicole Fleming; Scott Magness; Li Qian; Jiandong Liu

doi:10.3791/53877

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분리 및 Zebrafish의 배아에서 단일 세포의 특성

Isolation and Characterization of Single Cells from Zebrafish Embryos

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Isolation and Characterization of Single Cells from Zebrafish Embryos

분리 및 Zebrafish의 배아에서 단일 세포의 특성

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14,430 Views

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09:25 min

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March 12, 2016

DOI:

10.3791/53877-v

DOI:

10.3791/53877-v

•

09:25 min

March 12, 2016

•

14 Views

Leigh Ann Samsa^1,2, Nicole Fleming^2,3, Scott Magness¹, Li Qian^2,3, Jiandong Liu^2,3

¹Department of Cell Biology and Physiology,University of North Carolina at Chapel Hill, ²McAllister Heart Institute,University of North Carolina at Chapel Hill, ³Department of Pathology and Laboratory Medicine,University of North Carolina at Chapel Hill

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Summary

This protocol describes a method for isolating single cells from zebrafish embryos, enriching for cells of interest, capturing zebrafish cells in microfluidic based single cell multiplex systems, and assessing gene expression from single cells.

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Single Cell Isolation Zebrafish Embryos Microfluidic-based Single-cell Analysis Gene Expression 발생학 Cell Heterogeneity Cell Dissociation Cell Preparation Chorion Removal De-yolking Cell Washing Cell Resuspension

분리 및 Zebrafish의 배아에서 단일 세포의 특성

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