Differentiation of Atrial Cardiomyocytes from Pluripotent Stem Cells Using the BMP Antagonist Grem2

Jeffery B. Bylund; Antonis K. Hatzopoulos

doi:10.3791/53919

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BMP 길항제 Grem2를 사용하여 다 능성 줄기 세포에서 심방 심근의 차별화

Differentiation of Atrial Cardiomyocytes from Pluripotent Stem Cells Using the BMP Antagonist Grem2

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Differentiation of Atrial Cardiomyocytes from Pluripotent Stem Cells Using the BMP Antagonist Grem2

BMP 길항제 Grem2를 사용하여 다 능성 줄기 세포에서 심방 심근의 차별화

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7,700 Views

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08:52 min

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March 10, 2016

DOI:

10.3791/53919-v

DOI:

10.3791/53919-v

•

08:52 min

March 10, 2016

•

1 Views

Jeffery B. Bylund^1,2, Antonis K. Hatzopoulos^1,3

¹Department of Medicine, Division of Cardiovascular Medicine,Vanderbilt University School of Medicine, ²Department of Pharmacology,Vanderbilt University, ³Department of Cell & Developmental Biology,Vanderbilt University School of Medicine

내레이션 대본

Summary

Generating cardiomyocytes from pluripotent stem cells in vitro allows access to large amounts of cardiac tissue in vitro for basic science and clinical applications. This protocol uses the atrializing factor Grem2 to both increase the numbers of cardiomyocytes obtained and to generate cardiomyocytes with an atrial phenotype.