Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Nicolaas H. Fourie; Ralph M. Peace; Sarah K. Abey; LeeAnne B. Sherwin; John W. Wiley; Wendy A. Henderson

doi:10.3791/54693

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과민성 대장 증후군에서의 miRNAs 순환의 교란은 멀티 플렉스 높은 처리량 유전자 발현 플랫폼을 사용하여 감지

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

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JoVE 신문 유전학

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

과민성 대장 증후군에서의 miRNAs 순환의 교란은 멀티 플렉스 높은 처리량 유전자 발현 플랫폼을 사용하여 감지

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8,215 Views

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10:37 min

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November 30, 2016

DOI:

10.3791/54693-v

DOI:

10.3791/54693-v

•

10:37 min

November 30, 2016

•

9 Views

Nicolaas H. Fourie¹, Ralph M. Peace^1,2, Sarah K. Abey¹, LeeAnne B. Sherwin¹, John W. Wiley³, Wendy A. Henderson¹

¹Digestive Disorders Unit, National Institute of Nursing Research,National Institutes of Health, DHHS, ²National Institutes of Health Research Scholar,Howard Hughes Medical Institute, ³Internal Medicine, Medical School,University of Michigan

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Summary

We describe the use of a multiplexed high-throughput gene expression platform that quantitates gene expression by barcoding and counting molecules in biological substrates without the need for amplification. We used the platform to quantitate microRNA (miRNA) expression in whole blood in subjects with and without irritable bowel syndrome.