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Developmental Biology

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Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish
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Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish

Article DOI: 10.3791/54820-v • 07:19 min • December 21st, 2016
December 21st, 2016 •
1, 1, 1, 1
1Department of Life Science, Tunghai University

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요약
Automatic Translation

The interrenal gland in zebrafish is the teleostean counterpart of the mammalian adrenal gland. This protocol introduces how to perform the 3-β-hydroxysteroid dehydrogenase (Δ5-4 isomerase; 3β-Hsd) enzymatic activity assay, which detects differentiated steroidogenic cells in the developing zebrafish.

Tags

Interrenal Steroidogenic Cells Zebrafish Enzymatic Activity Assay Staining Procedure Genetic Or Chemical Screens Fluorescent Reporter Lines Kidney Blood Vessels Vibratome Sectioning Immunohistochemistry Confocal Microscopy Beta-Hsd Activity Staining Developing Zebrafish Embryos PTU Dechorionated Embryos PFA PBST
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