Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay

Xinzhou Zhu; Andrea Zelmer; Sven Wellmann

doi:10.3791/55218

Journal

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생화학

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공동 면역 침전에 의한 핵 및 세포질 분수에서 단백질 - 단백질 상호 작용의 시각화 및 현장에서 근접 내고 분석

Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and <em>In Situ</em> Proximity Ligation Assay

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JoVE 신문 생화학

Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay

공동 면역 침전에 의한 핵 및 세포질 분수에서 단백질 - 단백질 상호 작용의 시각화 및 현장에서 근접 내고 분석

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12,724 Views

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10:05 min

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January 16, 2017

DOI:

10.3791/55218-v

DOI:

10.3791/55218-v

•

10:05 min

January 16, 2017

•

12 Views

Xinzhou Zhu¹, Andrea Zelmer¹, Sven Wellmann^1,2

¹University Children’s Hospital Basel (UKBB),University of Basel, ²Department of Clinical Research,University of Basel

내레이션 대본

Summary

Protein-protein interactions can occur in both the nucleus and the cytoplasm of a cell. To investigate these interactions, traditional co-immunoprecipitation and modern proximity ligation assay are applied. In this study, we compare these two methods to visualize the distribution of NF90-RBM3 interactions in the nucleus and the cytoplasm.