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Development of a Refined Protocol for Trans-scleral Subretinal Transplantation of Human Retinal Pigment Epithelial Cells into Rat Eyes

Article DOI: 10.3791/55220-v • 12:45 min • August 12th, 2017
August 12th, 2017 •
1, 1, 1, 1, 1, *1, *1
1Neural Stem Cell Institute
* These authors contributed equally

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Subretinal injection has been widely applied in preclinical studies of stem cell replacement therapy for age-related macular degeneration. In this visualized article, we describe a less risky, reproducible and precisely modified subretinal injection technique via the trans-scleral approach to deliver cells into rat eyes.

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Trans-scleral Subretinal Transplantation Refined Protocol Human Retinal Pigment Epithelial Cells Rat Eyes Reproducible Technique Minimal Risk Retina Damage Temporal Conjunctiva Limbus Sclera Beveled 31-gauge Insulin Needle Pilot Hole Beveled 33-gauge Injection Needle Syringe Specific Angle And Depth Injection Hole Self Seals Degenerative Retinopathies Age-related Macular Degeneration AMD Retinal Pigmented Epithelial Cells Loss Retinal Fluororeceptor Dysfunction
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