<em>In Vitro</em> Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

Jianle Wang; Montserrat C. Anguera

doi:10.3791/55268

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인간 다 능성의 체외 분화에서 융모 세포로 줄기 세포

<em>In Vitro</em> Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

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In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

인간 다 능성의 체외 분화에서 융모 세포로 줄기 세포

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8,555 Views

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08:21 min

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March 16, 2017

DOI:

10.3791/55268-v

DOI:

10.3791/55268-v

•

08:21 min

March 16, 2017

•

1 Views

Jianle Wang¹, Montserrat C. Anguera¹

¹Department of Biomedical Sciences, School of Veterinary Medicine,University of Pennsylvania

내레이션 대본

Summary

Here, we present a protocol to efficiently generate human trophoblastic cells from human pluripotent stem cells using bone morphogenic protein 4 and inhibitors of the Activin/Nodal pathways. This method is suitable for the efficient differentiation of human pluripotent stem cells and can generate large quantities of cells for genetic manipulation.