10,681 Views
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09:27 min
March 04, 2017
DOI:
10.3791/55333-v
PCR 매개 유전자 변형은 시각화 및 효모 세포 및 단백질의 정량을 용이 칸디다 종의 형광 단백질 융합체를 생성하기 위해 사용될 수있다. 여기서, 우리는 칸디다에서 형광 단백질 융합 (Eno1-FP)을 구축하기위한 전략을 제시한다.
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Cite this Article
Gonia, S., Berman, J., Gale, C. A. Generation of Fluorescent Protein Fusions in Candida Species. J. Vis. Exp. (121), e55333, doi:10.3791/55333 (2017).
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