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Journal / Developmental Biology / In Vivo Imaging af transgene genekspression i…
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Developmental Biology

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JoVE Journal Developmental Biology
In Vivo Imaging af transgene genekspression i Individuel Retinal progenitorer i kimære zebrafisk embryoner til Studieinformationen Cell Nonautonomous påvirkninger
Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects
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In Vivo Imaging af transgene genekspression i Individuel Retinal progenitorer i kimære zebrafisk embryoner til Studieinformationen Cell Nonautonomous påvirkninger

Article DOI: 10.3791/55490-v • 10:36 min • March 22nd, 2017
March 22nd, 2017 •
1,2, 2, 3, 1,2
1School of Biosciences, The University of Melbourne, 2Australian Regenerative Medicine Institute (ARMI), Monash University, 3The David J Apple Center for Vision Research, Department of Ophthalmology, Heidelberg University

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Live-sporing af individuelle WT retinale stamfædre i distinkte genetiske baggrunde giver mulighed for vurdering af bidraget fra celle non-autonome signalering under neurogenese. Her blev en kombination af gen knockdown, kimære generation via embryo transplantation og in vivo time-lapse konfokal billeddannelse anvendes til dette formål.

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Developmental Biology zebrafisk transplantation kimære levende billeddannelse celle non-autonome nethinde genekspression timing neurogenese
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