Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Jonas Gross; Nicolas Wirth; Ingrid Tessmer

doi:10.3791/55501

Journal

/

유전학

/

핵 포스 현미경 검사에 의한 DNA 절편 검사에서의 DNA 병변 인식 연구

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

JoVE 신문
유전학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 유전학

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

핵 포스 현미경 검사에 의한 DNA 절편 검사에서의 DNA 병변 인식 연구

Please note that all translations are automatically generated. Click here for the English version.

9,342 Views

•

10:59 min

•

May 24, 2017

DOI:

10.3791/55501-v

DOI:

10.3791/55501-v

•

10:59 min

May 24, 2017

•

1 Views

Jonas Gross*¹, Nicolas Wirth*¹, Ingrid Tessmer¹

¹Rudolf Virchow Center for Experimental Biomedicine,University of Würzburg

내레이션 대본

Summary

Here, the study of different DNA lesion recognition approaches via single molecule AFM imaging is demonstrated with the nucleotide excision repair system as an example. The procedures of DNA and protein sample preparations and experimental as well as analytical details for the AFM experiments are described.