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JoVE Journal Developmental Biology
Overdracht van Mammary Gland-vormende Vermogen Tussen Mammary Basal Epithelial Cellen en Mammary Luminale Cellen via Extracellulaire Vesicles / Exosomen
Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage
Journal (Developmental Biology)
Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage
Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence
Journal (Developmental Biology)
Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence
 
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Overdracht van Mammary Gland-vormende Vermogen Tussen Mammary Basal Epithelial Cellen en Mammary Luminale Cellen via Extracellulaire Vesicles / Exosomen

Article DOI: 10.3791/55736-v • 09:59 min • June 3rd, 2017
June 3rd, 2017 •
*1, *1, 1, 1, 1
1Institute of Cellular and System Medicine, National Health Research Institutes
* These authors contributed equally

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Dit protocol beschrijft methoden voor het zuiveren, kwantificeren en karakteriseren van extracellulaire vesicles (EVs) / exosomen van niet-aanhechtende / mesenchymale mammary epitheelcellen en voor het gebruik ervan om het vermogen van de borstkanker te overdragen naar luminale mammary epitheelcellen. EV's / exosomen afgeleid van stamachtige mammale epitheelcellen kunnen deze celeigenschap overbrengen aan cellen die de EV's / exosomen innemen.

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Ontwikkelingsbiologie nummer 124 extracellulaire blaasjes exosoom stamcel nanodeeltjes tracking analyse ultracentrifugatie borstkanker dichtheidsgradiënt
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