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Optical Cross-Sectional Muscle Area Determination of Drosophila Melanogaster Adult Indirect Flight Muscles

Article DOI: 10.3791/56179-v • 06:43 min • March 31st, 2018
March 31st, 2018 •
1,2,3, 1,2,3, 1,2,3
1Department of Genetics and Interdisciplinary Research Structure for Biotechnology and Biomedicine (BIOTECMED), University of Valencia, 2Incliva Health Research Institute, 3Joint unit CIPF-Incliva

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We report a method to quantify muscle area, which is an indirect method to determine muscle mass in Drosophila adults. We demonstrate the application of our methodology by analyzing the indirect flight muscles in a Drosophila model of Myotonic Dystrophy disease.

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Optical Cross-sectional Muscle Area Determination Drosophila Melanogaster Adult Indirect Flight Muscles Muscle Mass Quantification Muscle Atrophy Dorsal Longitudinal Muscles Fixation And Resin Embedding CO2 Anesthesia Leg And Wing Removal Abdomen Removal Carcass Transfer Solution One Solution Two Incubation On Ice Solution Removal Dehydration Graded Ethanol Series Propylene Oxide Incubation Resin And Propylene Oxide Mixture
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